Recombinant Rat Lactoylglutathione lyase (Glo1)

Code CSB-YP740739RA
MSDS
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Source Yeast
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Code CSB-EP740739RA
MSDS
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Source E.coli
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Code CSB-EP740739RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740739RA
MSDS
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Source Baculovirus
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Code CSB-MP740739RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Glo1
Uniprot No.
Alternative Names
Glo1Lactoylglutathione lyase; EC 4.4.1.5; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase
Species
Rattus norvegicus (Rat)
Expression Region
2-184
Target Protein Sequence
AEPQPASSG LTDEAALSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPSMKFSL YFLAYEDKND IPKDKTERTA WAFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYEACK RFEELGVKFV KKPDDGKMKG LAFVQDPDGY WIEILNPNKM ATII
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B.
Gene References into Functions
  1. Diabetes-induced impairment of arteriogenic adaptation can be partially rescued by overexpressing of GLO-I, indicating a role of AGEs in diabetes-induced impaired collateral formation PMID: 27296676
  2. These results show the importance of Glo-I as major detoxifying enzyme for methylglyoxal in cirrhosis. The reduced expression of Glo-I in cirrhosis demonstrates a possible explanation for increased inflammatory injury and suggests a "vicious circle" in liver disease. PMID: 28231326
  3. Increased biosynthesis of collagens 1 and 4 was detected at mRNA and protein level following knockdown of glyoxalase 1 in L6 myoblasts. PMID: 28241483
  4. Data suggest that the high dynamics of glyoxalase 1 expression and distribution following neuronal injury may indicate a role of Glyoxalase 1. PMID: 24498315
  5. ovarian GLO-I activity may be regulated by dietary composition and androgen levels. PMID: 22859292
  6. Lower glyoxalase 1 activity in the neuronal tissues was associated with a higher blood glucose concentration. PMID: 22893478
  7. Renal ischemia reperfusion-induced tubulointerstitial injury was associated with a deterioration in renal glyoxalase I activity. PMID: 19211689

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Protein Families
Glyoxalase I family
Database Links
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