Recombinant Rat Nuclear receptor subfamily 0 group B member 1 (Nr0b1)

Code CSB-YP016041RA
MSDS
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Source Yeast
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Code CSB-EP016041RA
MSDS
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Source E.coli
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Code CSB-EP016041RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016041RA
MSDS
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Source Baculovirus
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Code CSB-MP016041RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nr0b1
Uniprot No.
Alternative Names
Nr0b1; Ahch; Dax1; Nuclear receptor subfamily 0 group B member 1; Nuclear receptor DAX-1
Species
Rattus norvegicus (Rat)
Expression Region
1-472
Target Protein Sequence
MAGEDHPWHG SILYNLLMSA KQKHGSREER EVRLGAQCWG CACGTQPVLG GEGLPGGQAL SLLYRCCFCG ENHPRQGGIL YSMLTNARQP SGATEAPRAR FRTPCWGCAC SNAKPLVGRX GLPAGQVPSL LYRCCFCGKK HPRQGSILYS LLTNAQQTHV SREVPEAHRG GEWWQLSYCT HNVGGPEGLQ STQAMAFLYR SYVCCEEQPQ QSSVASDTPV RADQTPAAPQ EQPRAPWWDT SSGVQRPIAL KDPQVVCEAA SAGLLKTLRF VKYLPCFQIL PLDQQLVLVR SCWAPLLMLE LAQDHLHFEM MEISEPNLMH EMLTTRRQET EGPEPADPQA TEQPQTVSAE AGHVLSVAAV QAIKSFFFKC WSLNIDTKEY AYLKGTVLFN PDLPGLQCVK YIESLQWRTQ QILTEHIRLM QREYQIRSAE LNSALFLLRF INTDVVTELF FRPIIGAVSM DDMMLEMLCA KL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Gene References into Functions
  1. In conclusion, the presented data revealed that the pituitary Nr5a1/Ctnnb1/Nr0b1 system is controlled by endogenous Gnrh network activity, and hypothalamic-derived regulatory inputs can affect the processes responsible for gene transcription, translation, and protein intracellular translocation. PMID: 30342431
  2. High fat diet aggravates BDE47-inhibited testosterone production through hyperinsulinemia, and the accumulation of testicular BDE47 that induces the up-regulation of DAX-1 and the subsequent down-regulation of steroidogenic proteins. PMID: 28300557
  3. In vivo blockade of testicular alpha1-adrenergic receptors changed the stress-induced decrease of Nur77 and increase of Arr19 and Dax1 in Leydig cells. PMID: 26003139
  4. These results suggest that the low level of DAX-1 may enhance the transcriptional activity of SF-1 and expression of genes related to glucocorticoid synthesis, which are targeted by SF-1, in adrenals from SHR. PMID: 22447829
  5. DAX-1 acts as a novel corepressor of liver X receptor alpha and inhibits hepatic lipogenesis PMID: 20080977
  6. Nr0b1 negatively regulates steroid synthesis through the inhibition of StAR expression and indicate that the absence of Nr0b1 in R2C cells is, at least in part, responsible for the constitutive steroidogenesis and StAR expression observed. PMID: 15358680
  7. Dexamethasone increases expression of DAX-1, which results in increased amounts of complexed DAX-1-SF-1, in the absence of any change in the expression of SF-1. PMID: 17526944

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Nuclear hormone receptor family, NR0 subfamily
Database Links
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