Recombinant Rat Nucleus accumbens-associated protein 1 (Nacc1)

Code CSB-YP015407RA
MSDS
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Source Yeast
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Code CSB-EP015407RA
MSDS
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Source E.coli
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Code CSB-EP015407RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015407RA
MSDS
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Source Baculovirus
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Code CSB-MP015407RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nacc1
Uniprot No.
Alternative Names
Nacc1; Btbd14b; Nac1; Nucleus accumbens-associated protein 1; NAC-1; BTB/POZ domain-containing protein 14B
Species
Rattus norvegicus (Rat)
Expression Region
1-514
Target Protein Sequence
MAQTLQMEIP NFGNSILECL NEQRLQGLYC DVSVVVKGHA FKAHRAVLAA SSSYFRDLFN SSRSAVVELP AAVQPQSFQQ ILTFCYTGRL SMNMGDQFLL IYTAGFLQIQ EIMEKGTEFF LKVSSPSCDS QGLHPEEAPS SEPQSPVAQI LGWPACSTPL PLVSRVKTEQ ELDSVQCTPM AKRLWDSSQK EAGGSGGNNG SRKMAKFSTP DLAPNRMPQP VSVATATAAV AVVAVGGCVS GPSMSERTSP GTSSAYTSDS PSSYHNEEDE EEDAGEEGTD EQYRQICNMY TMYSMLNVGQ TVEKVEALPE QVVLESHSRI RVRQDLASLP AELINQIGNR CHPKLYDEGD PSEKLELVTG TNVYITRAQL MNCHVSAGTR HKVLLRRLLA SFFDRNTLAN SCGTGIRSST NDPRRKPLDS RVLHAVKYYC QNFAPNFKES EMNAIAADMC TNARRVVRKS WLPKTKPLHL VEGDNYSSFI SDTGKIEPDM MSMEHSFETA SHDGEAGPSA EVLQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as a transcriptional repressor. Isoform 1 is a stronger transcriptional repressor than isoform 2. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Gene References into Functions
  1. The two isoforms of NAC-1 may differentially affect neuronal functions, including the regulation of cocaine-induced locomotion. The longer isoform was the more abundant of the two NAC1 proteins and the ratio between them differed throughout the rat brain. PMID: 11906783
  2. aberrantly regulated NAC1 expression in the mammalian brain may contribute to programmed cell death PMID: 12725910
Subcellular Location
Nucleus. Cytoplasm. Note=Punctate distribution in nucleus. In differentiated PC12 cells diffusely distributed in the cytoplasm and is dependent on phosphorylation.
Tissue Specificity
Highly expressed in the hippocampus, brain cortex, cerebellum and brainstem. Expressed in the nucleus accumbens, olfactory tubercle, the striatum, frontal and parietal cortex and ventral pallidum. Weakly expressed in the heart, liver, kidney, spleen, test
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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