Recombinant Rat Paired box protein Pax-4 (Pax4)

Code CSB-YP017490RA
MSDS
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Source Yeast
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Code CSB-EP017490RA
MSDS
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Source E.coli
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Code CSB-EP017490RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017490RA
MSDS
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Source Baculovirus
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Code CSB-MP017490RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pax4
Uniprot No.
Alternative Names
Pax4Paired box protein Pax-4
Species
Rattus norvegicus (Rat)
Expression Region
1-349
Target Protein Sequence
MQQDGLSSVN QLGGLFVNGR PLPLDTRQQI VQLAIRGMRP CDISRSLKVS NGCVSKILGR YYRTGVLEPK GIGGSKPRLA TPAVVARIAQ LKDEYPALFA WEIQRQLCAE GLCTQDKAPS VSSINRVLRA LQEDQRLHWT QLRSPAVLAP ALPSPHSNCE APRGPHPGTS HRNRTIFSPG QAEALEKEFQ RGQYPDSVVR GKLAAATSLP EDTVRVWFSN RRAKWRRQEK LKWETQMPGA SQDLMVPKDS PGIISAQQSP GSVPSAALPV LEQLNPSFCQ LCWGAVPDRC SSDTTSQACL QPYWECHSLL PVASSSYMEF AWPCLTTHPV HHLIGGPGQA PSTYYLHWP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that competes with PAX6 in binding to a common element in the glucagon, insulin and somatostatin promoters.
Gene References into Functions
  1. The Pax4 transcripts were detected in adult rat islets, and levels were induced by the mitogens activin A and betacellulin. PMID: 15596543
  2. activation of Pax4 in Pdx1-VP16-expressing cells reprograms pancreatic precursor-like WB-1 cells into glucose-responsive, more mature insulin-producing cells PMID: 16732298
  3. Causal association between Pax4 mRNA levels and susceptibility of rat insulinoma cells to apoptosis suggests an important physiological function for Pax4 in cell survival. PMID: 17260022
  4. The daily changes in Pax4 expression may influence gene expression in the pineal gland. PMID: 18818287
  5. The daily rhythm in pineal Pax4 expression is controlled by a neural system which acts on pinealocytes through an adrenergic-cyclic AMP signalling mechanism. PMID: 18818287
  6. Pineal expression of Pax4 is undetectable prior to birth and increases thereafter; in the adult there is a daily rhythm in Pax4 expression with high levels during the day. PMID: 18818287
  7. Retinal expression of Pax4 is undetectable prior to birth but increases thereafter; in the adult there are daily changes which may influence retinal gene expression PMID: 19012751

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family
Tissue Specificity
Specifically expressed in pancreatic islets.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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