Recombinant Rat Pituitary-specific positive transcription factor 1 (Pou1f1)

Code CSB-YP018391RA
MSDS
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Source Yeast
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Code CSB-EP018391RA
MSDS
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Source E.coli
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Code CSB-EP018391RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018391RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018391RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pou1f1
Uniprot No.
Alternative Names
Pou1f1; Ghf-1; Pit-1; Pit1; Pituitary-specific positive transcription factor 1; PIT-1; Growth hormone factor 1; GHF-1
Species
Rattus norvegicus (Rat)
Expression Region
1-291
Target Protein Sequence
MSCQPFTSAD TFIPLNSDAS AALPLRMHHS AAEGLPASNH ATNVMSTATG LHYSVPSCHY GNQPSTYGVM AGTLTPCLYK FPDHTLSHGF PPLHQPLLAE DPTASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV GALYNEKVGA NERKRKRRTT ISIAAKDALE RHFGEHSKPS SQEIMRMAEE LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
Gene References into Functions
  1. Upregulation of Pit-1 protein is associated with Vascular Calcification. PMID: 27840385
  2. Down-regulation of Pit-1 reduced prolactin levels during pulse periods. PMID: 26875730
  3. Blocking expression of endogenous PIT-1 induced a marked decrease of cell proliferation in somatolactotroph cells. PMID: 25822178
  4. Results suggest a sexually dimorphic response of lactotroph cells to the proliferative effects of EGF, with the PKCepsilon/ERK1/2 Pit-1 pathway being involved in this action. PMID: 21075822
  5. CEBPD and Pit1 interact and attenuate each other's binding to the PRL promoter. PMID: 21980073
  6. Expression of parathyroid Pit-1 in hyperphosphatemia-induced SHPT of CRF rats was upregulated. Pit-1 may mediate the stimulation to parathyroid gland by hyperphosphatemia. PMID: 20819513
  7. Data suggest phosphorylation of Pit-1 (Pou1f1) at T220 serves as a mechanism to modulate Pit-1 target genes by regulating binding of Pit-1 to monomeric (Pit-1) versus dimeric (Ets-1/Pit-1) sites on DNA PMID: 19887646
  8. full responsiveness to several signaling pathways regulating the hPRL promoter requires the B2 Pitx binding site and that Pitx factors may be part of the proteic complex involved in these regulations PMID: 12223489
  9. estrogen receptor alpha (responsible for terp-1) promoter regulation involves ERE and Pit-1 cis-elements and corresponding trans-acting factors PMID: 12810539
  10. the smad pathway and the tumor suppressor menin are key regulators of activin effects on PRL and Pit-1 expression, as well as on cell growth inhibition PMID: 15031321
  11. Pit-1 induces the transient differentiation of hepatic progenitor cells into prolactin-producing cells. PMID: 15637144
  12. The redistribution of co-repressor complexes by Pit-1 might represent an important mechanism by which transcription factors direct changes in cell-specific gene expression. PMID: 16030140
  13. These results suggest that a diet high in soybean products modulates anterior pituitary hormone production by regulating Pit-1 induction, in iodine-deficient animals. PMID: 16200459
  14. These data suggest that Pit-1beta represses hormone gene expression by heterodimerizing with Pit-1 and interfering with the assembly of the Pit-1-CBP complex required for prolactin promoter activity in pituitary cells. PMID: 16216912
  15. These data support a model in which the Pit-1 binding sites in the human growth hormone (hGH) locus control region (LCR) allosterically program the bound Pit-1 complex for chromatin activating functions. PMID: 16914737
  16. the Pit-1/Pit-1beta TADs are composed of multiple, modular, and transferable subdomains, including a regulatory R1 domain, a basal activation region, a selective inhibitory-Ras-responsive segment, and a beta-specific repressor domain PMID: 17021049
  17. Regulates growth hormone-releasing hormone receptor (Ghrhr) gene. PMID: 17536003
  18. Brn-5 enhances prolactin gene expression directly in association with Pit-1 and CREB-binding protein, and indirectly via the activation of Pit-1 gene expression. PMID: 17933456
  19. These data suggest that the regulation of cell proliferation in response to a specific growth factor depends in certain cell populations on the presence of a tissue-specific transcription factor. PMID: 17941086
  20. In the embryonic period, PROP1 coexists transiently with PIT1, which is the anterior pituitary-specific factor and is a target of PROP1, but not any hormones. PMID: 19442651
  21. analysis of a Pit-1beta 26-amino acid dominant and independent repressor domain PMID: 19556346
  22. Activation of Syt1 gene expression is part of a mechanism mediating POU1F-induced differentiation of pituitary cells. PMID: 19608642

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Subcellular Location
Nucleus.
Protein Families
POU transcription factor family, Class-1 subfamily
Database Links

UniGene: Rn.10445

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