Recombinant Rat Protein CYR61(Cyr61)

Code CSB-YP863670RA
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Source Yeast
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Code CSB-EP863670RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863670RA
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Source Baculovirus
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Code CSB-MP863670RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Cyr61
Uniprot No. Q9ES72
Alternative Names Ccn1; Cyr61; Igfbp10CCN family member 1; Cellular communication network factor 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein CYR61
Species Rattus norvegicus (Rat)
Expression Region 25-379
Target Protein Sequence TCPAAC HCPLEAPKCA PGVGLVRDGC GCCKVCAKQL NEDCSKTQPC DHTKGLECNF GASSTALKGI CRAQSEGRPC EYNSRIYQNG ESFQPNCKHQ CTCIDGAVGC IPLCPQELSL PNLGCPNPRL VKVSGQCCEE WVCDEDSIKD SLDDQDDLLG FDASEVELTR NNELIAIGKG SSLKRLPVFG TEPRVLYNPL HAHGQKCIVQ TTSWSQCSKS CGTGISTRVT NDNSECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKS PEPVRFTYAG CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEM FSKNVMMIQS CKCNYNCPHP NEASFRLYSL FNDIHKFRD
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Gene References into Functions
  1. Compared with wild rats, Cyr61 was overexpressed in the lung tissue, pulmonary arterial and PASMCs in PAH rats. Exogenous recombinant Cyr61 protein promoted the proliferation of PASMCs in a dose-dependent manner. While the expression of Cyr61 in PASMCs was inhibited by specific siRNA, cell proliferation was restrained and the expression of p-AKT declined PMID: 28824319
  2. the matricellular protein CCN1 gene transfer induces hepatic stellate cell apoptosis through endoplasmic reticulum stress and unfolded protein response PMID: 26515130
  3. Cyclic AMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1. PMID: 25446180
  4. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
  5. In line with dermal fibrosis and scar formation, CCN1/CYR61 is involved in liver injury repair and tissue remodeling. CCN1/CYR61 gene transfer into extracellular matrix-producing liver cells is therefore potentially beneficial in liver fibrotic therapy PMID: 24487063
  6. Muscle progenitor cells treated with aging rodent serum caused CCN1 mRNA to increase by seven fold. PMID: 24196529
  7. Report assays with site-direct mutation analysis of CYR61/CCN1 promoter demonstrated that MRTF-A transactivated CYR61/CCN1 promoter mainly depending on CArG box. PMID: 23624342
  8. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
  9. Cyr61, an angiogenic factor with previously unknown neuronal function, is a novel regulator of dendritic growth. PMID: 23362279
  10. CYR-61 is important in the pathogenesis of rheumatoid arthritis, and SIRT-1/FoxO3a signaling is crucial to induction of CYR-61 in synovial fibroblasts. PMID: 23239110
  11. Endogenous CCN1 regulates proliferation of VSMCs and neointimal hyperplasia. Inhibition of CCN1 may provide a promising strategy for the prevention of restenosis after vascular interventions [CCN1]. PMID: 18388330
  12. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
  13. Suggest that CCN1, from the microenvironment of injured vessels, enhances reendothelialization via a direct action on endothelial progenitor cell differentiation, revealing a possible new mechanism underlying the process of vascular repair. PMID: 20830586
  14. Cyr61 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells. PMID: 20458273
  15. CYR61 is synthesized in arterial smooth muscle walls during proliferative restenosis PMID: 11897702
  16. induction of cyr61 is important for neuronal cell death in the central nervous system hippocampal progenitor cells, and JNK activation, but not of p38, as well as the subsequent SRF phosphorylation are involved in cyr61 gene induction PMID: 12576482
  17. after severe glomerular damage in anti-Thy-1 glomerulonephritis, Cyr61 may be involved in glomerular remodeling as a factor secreted from podocytes to inhibit mesangial cell migration. PMID: 12707386
  18. Overexpression of the cyr61 gene in stably transfected rat SMC (smooth muscle cells) line A7r5 inhibited the proliferation and DNA and protein synthesis. Cyr61 can also act as a growth inhibitor in SMC of genetically hypertensive rats. PMID: 12861037
  19. In a rat balloon carotid arterial injury model, CYR61 was rapidly induced in VSMCs in the early stage of injury and remained elevated until 14 days. PMID: 17234971
  20. The dissimilar growth characteristics between high and low Cx43 expressors may be due to differential regulation of CCN3 by varying levels of Cx43 PMID: 18004727
  21. Data suggest that matrix protein CCN1 may play an important role in microenvironment-mediated biological properties of endothelial progenitor cells . PMID: 18755182
  22. THBS1, Cyr61 and CTGF were all over-expressed in esophageal squamous cell carcinoma. PMID: 19698122
  23. Cyr61 is an early marker of extracellular matrix remodeling in rat bladder cells PMID: 12217894

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Subcellular Location Secreted.
Protein Families CCN family
Database Links

KEGG: rno:83476

STRING: 10116.ENSRNOP00000019501

UniGene: Rn.22129

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