Rat Protein CYR61(CYR61) ELISA kit

Instructions
Code CSB-EL006463RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cysteine-rich, angiogenic inducer, 61
Alternative Names Ccn1 ELISA kit; Cyr61 ELISA kit; Igfbp10CCN family member 1 ELISA kit; Cellular communication network factor 1 ELISA kit; Cysteine-rich angiogenic inducer 61 ELISA kit; Insulin-like growth factor-binding protein 10 ELISA kit; IBP-10 ELISA kit; IGF-binding protein 10 ELISA kit; IGFBP-10 ELISA kit; Protein CYR61 ELISA kit
Abbreviation CYR61
Uniprot No. Q9ES72
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat CYR61 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %87-97
1:2Average %95
Range %91-99
1:4Average %98
Range %96-101
1:8Average %99
Range %96-104
Recovery
The recovery of rat CYR61 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9892-102
EDTA plasma (n=4)9690-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.625 2.529 2.577 2.413
10002.062 1.956 2.009 1.845
5001.384 1.366 1.375 1.211
2500.871 0.881 0.876 0.712
1250.551 0.539 0.545 0.381
62.50.342 0.351 0.347 0.183
31.250.249 0.255 0.252 0.088
00.164 0.163 0.164  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).
Gene References into Functions
  1. Compared with wild rats, Cyr61 was overexpressed in the lung tissue, pulmonary arterial and PASMCs in PAH rats. Exogenous recombinant Cyr61 protein promoted the proliferation of PASMCs in a dose-dependent manner. While the expression of Cyr61 in PASMCs was inhibited by specific siRNA, cell proliferation was restrained and the expression of p-AKT declined PMID: 28824319
  2. the matricellular protein CCN1 gene transfer induces hepatic stellate cell apoptosis through endoplasmic reticulum stress and unfolded protein response PMID: 26515130
  3. Cyclic AMP-induced actin-cytoskeleton remodelling regulates expression of CCN1 through MKL1. PMID: 25446180
  4. These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095
  5. In line with dermal fibrosis and scar formation, CCN1/CYR61 is involved in liver injury repair and tissue remodeling. CCN1/CYR61 gene transfer into extracellular matrix-producing liver cells is therefore potentially beneficial in liver fibrotic therapy PMID: 24487063
  6. Muscle progenitor cells treated with aging rodent serum caused CCN1 mRNA to increase by seven fold. PMID: 24196529
  7. Report assays with site-direct mutation analysis of CYR61/CCN1 promoter demonstrated that MRTF-A transactivated CYR61/CCN1 promoter mainly depending on CArG box. PMID: 23624342
  8. Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676
  9. Cyr61, an angiogenic factor with previously unknown neuronal function, is a novel regulator of dendritic growth. PMID: 23362279
  10. CYR-61 is important in the pathogenesis of rheumatoid arthritis, and SIRT-1/FoxO3a signaling is crucial to induction of CYR-61 in synovial fibroblasts. PMID: 23239110
  11. Endogenous CCN1 regulates proliferation of VSMCs and neointimal hyperplasia. Inhibition of CCN1 may provide a promising strategy for the prevention of restenosis after vascular interventions [CCN1]. PMID: 18388330
  12. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
  13. Suggest that CCN1, from the microenvironment of injured vessels, enhances reendothelialization via a direct action on endothelial progenitor cell differentiation, revealing a possible new mechanism underlying the process of vascular repair. PMID: 20830586
  14. Cyr61 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells. PMID: 20458273
  15. CYR61 is synthesized in arterial smooth muscle walls during proliferative restenosis PMID: 11897702
  16. induction of cyr61 is important for neuronal cell death in the central nervous system hippocampal progenitor cells, and JNK activation, but not of p38, as well as the subsequent SRF phosphorylation are involved in cyr61 gene induction PMID: 12576482
  17. after severe glomerular damage in anti-Thy-1 glomerulonephritis, Cyr61 may be involved in glomerular remodeling as a factor secreted from podocytes to inhibit mesangial cell migration. PMID: 12707386
  18. Overexpression of the cyr61 gene in stably transfected rat SMC (smooth muscle cells) line A7r5 inhibited the proliferation and DNA and protein synthesis. Cyr61 can also act as a growth inhibitor in SMC of genetically hypertensive rats. PMID: 12861037
  19. In a rat balloon carotid arterial injury model, CYR61 was rapidly induced in VSMCs in the early stage of injury and remained elevated until 14 days. PMID: 17234971
  20. The dissimilar growth characteristics between high and low Cx43 expressors may be due to differential regulation of CCN3 by varying levels of Cx43 PMID: 18004727
  21. Data suggest that matrix protein CCN1 may play an important role in microenvironment-mediated biological properties of endothelial progenitor cells . PMID: 18755182
  22. THBS1, Cyr61 and CTGF were all over-expressed in esophageal squamous cell carcinoma. PMID: 19698122
  23. Cyr61 is an early marker of extracellular matrix remodeling in rat bladder cells PMID: 12217894

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Subcellular Location Secreted
Protein Families CCN family
Database Links

KEGG: rno:83476

STRING: 10116.ENSRNOP00000019501

UniGene: Rn.22129

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