Recombinant Rat Proton-coupled amino acid transporter 1 (Slc36a1), partial

Code CSB-YP852875RA
MSDS
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Source Yeast
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Code CSB-EP852875RA
MSDS
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Source E.coli
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Code CSB-EP852875RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP852875RA
MSDS
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Source Baculovirus
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Code CSB-MP852875RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc36a1
Uniprot No.
Alternative Names
Slc36a1; Lyaat1; Pat1; Proton-coupled amino acid transporter 1; Proton/amino acid transporter 1; Lysosomal amino acid transporter 1; LYAAT-1; Neutral amino acid/proton symporter; Solute carrier family 36 member 1
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Neutral amino acid/proton symporter. Probably involved in the efflux of lysosomal proteolysis products such as L-proline, L-alanine and glycine from the cell. May play a role in specifying sites for exocytosis in neurons.
Gene References into Functions
  1. Sertraline is an apparent non-competitive inhibitor of PAT1-mediated transport. PMID: 23962042
  2. knockdown of (PAT1) led to induced cellular growth, suggesting a role for PAT1 in regulating cellular proliferation of smooth muscle cells PMID: 24222668
  3. Expression of proton-assisted amino acid transporter 1 (PAT1) mRNA was slightly but not significantly induced by a single bout of exercise in soleus and extensor digitorum longus muscles. PMID: 24016666
  4. PAT1(Slc36a1) protein was present in rat rectal epithelium. PMID: 22234618
  5. The H+-coupled electrogenic lysosomal amino acid transporter LYAAT1 localizes to the axon and plasma membrane of hippocampal neurons. PMID: 12598615
  6. The essential role of chloride and fructose absorbing Slc36a1 is reported. PMID: 18496516

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
Protein Families
Amino acid/polyamine transporter 2 family
Tissue Specificity
Widely expressed and predominantly expressed in brain. Within the brain, expression restricted to neurons and not detected in glial cells. Abundant in regions rich in neurons using glutamate and GABA such as Purkinje cells in the cerebellum and pyramidal
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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