Recombinant Rat Putative RNA-binding protein 3 (Rbm3)

Code CSB-YP849766RA
MSDS
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Source Yeast
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Code CSB-EP849766RA
MSDS
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Source E.coli
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Code CSB-EP849766RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP849766RA
MSDS
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Source Baculovirus
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Code CSB-MP849766RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rbm3
Uniprot No.
Alternative Names
Rbm3; RNA-binding protein 3; RNA-binding motif protein 3
Species
Rattus norvegicus (Rat)
Expression Region
1-155
Target Protein Sequence
MSSEEGKLFV GGLNFNTDEQ ALEDHFSSFG PISEVVVVKD RETQRSRGFG FITFTNPEHA SDVMRAMNGE SLDGRQIRVD HAGKSARGTR GGAFGAHGRG RSYSRGGGDQ GYGSGRYDSR PGGYGYGYGR SRDYSGSQGG YDRYSGGNYR DNYDN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.
Gene References into Functions
  1. This study showed that a 1 degrees C reduction in temperature can induce pleiotropic biochemical changes by upregulating global protein synthesis in neurons. PMID: 26265550
  2. RBM3 expression was increased in astrocytes after spinal cord injury. RBM3 regulated proliferation of astrocytes in vitro. PMID: 24668366
  3. RBM3 might play important roles in central nervous system pathophysiology after spinal cord injury PMID: 24570111
  4. Aa cold-shock response may be part of a compensatory mechanism in muscles undergoing atrophy to preserve remaining muscle mass. RBM3, which was induced most highly in atrophic muscle from old rats, may be a therapeutic target to prevent muscle loss. PMID: 18753264
  5. These results indicate that expression of RBM3 is dynamically regulated in the developing brain and suggest that it contributes to translation-dependent processes underlying proliferation, differentiation, and plasticity. PMID: 19150436

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Subcellular Location
Nucleus. Cytoplasm. Cell projection, dendrite. Note=Localizes in mRNA granules in dendrites. Isoform 1 shows a much higher dendritic localization than isoform 2.
Tissue Specificity
Widely expressed in the brain. Highly expressed in the cerebellum and olfactory bulb (at protein level). Expressed in neurons and glial cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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