Recombinant Rat S-adenosylmethionine synthase isoform type-1 (Mat1a)

Code CSB-YP013516RA
MSDS
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Source Yeast
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Code CSB-EP013516RA
MSDS
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Source E.coli
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Code CSB-EP013516RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013516RA
MSDS
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Source Baculovirus
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Code CSB-MP013516RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mat1a
Uniprot No.
Alternative Names
Mat1a; Ams1S-adenosylmethionine synthase isoform type-1; AdoMet synthase 1; EC 2.5.1.6; Methionine adenosyltransferase 1; MAT 1; Methionine adenosyltransferase I/III; MAT-I/III
Species
Rattus norvegicus (Rat)
Expression Region
1-397
Target Protein Sequence
MNGPVDGLCD HSLSEEGAFM FTSESVGEGH PDKICDQISD AVLDAHLKQD PNAKVACETV CKTGMVLLCG EITSMAMIDY QRVVRDTIKH IGYDDSAKGF DFKTCNVLVA LEQQSPDIAQ CVHLDRNEED VGAGDQGLMF GYATDETEEC MPLTIVLAHK LNTRMADLRR SGVLPWLRPD SKTQVTVQYV QDNGAVIPVR VHTIVISVQH NEDITLEAMR EALKEQVIKA VVPAKYLDED TIYHLQPSGR FVIGGPQGDA GVTGRKIIVD TYGGWGAHGG GAFSGKDYTK VDRSAAYAAR WVAKSLVKAG LCRRVLVQVS YAIGVAEPLS ISIFTYGTSK KTERDELLEV VNKNFDLRPG VIVRDLDLKK PIYQKTACYG HFGRSEFPWE VPKKLVF
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.
Gene References into Functions
  1. The oncogene PDRG1 is an interaction target of methionine adenosyltransferases. PMID: 27548429
  2. Mat1a and Mthfr genes were downregulated, during early stages of rat 2-acetylaminofluorene-induced liver carcinogenesis. PMID: 22213190
  3. we found for the first time a post-transcriptional regulation of MAT1A and MAT2A by AUF1 and HuR in hepatocellular carcinoma. PMID: 22318685
  4. Data support a role for AUF1 and HuR/methyl-HuR in liver de-differentiation and development through the posttranslational regulation of MAT1A and MAT2A mRNAs. PMID: 20102719
  5. role of an intrasubunit disulfide in the association state; residues involved in this disulfide have been identified by mass spectrometry and using a set of single cysteine mutants as cysteines 35 and 61 PMID: 12496263
  6. methionine adenosyltransferase complexes by a catalytic mechanism that relies on Lys182 in the correct positioning of the substrates PMID: 12888348
  7. In this work, methionine adenosyltransferase I/III was identified in most rat tissues, both in the cytoplasm and the nucleus. PMID: 19497982

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Protein Families
AdoMet synthase family
Tissue Specificity
Detected in liver (at protein level). Expressed in liver.
Database Links
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