Recombinant Rat Selenocysteine lyase (Scly)

Code CSB-YP020827RA
MSDS
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Source Yeast
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Code CSB-EP020827RA
MSDS
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Source E.coli
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Code CSB-EP020827RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020827RA
MSDS
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Source Baculovirus
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Code CSB-MP020827RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Scly
Uniprot No.
Alternative Names
Scly; Selenocysteine lyase; EC 4.4.1.16
Species
Rattus norvegicus (Rat)
Expression Region
1-432
Target Protein Sequence
MDVARNGARG SVESPPNRKV YMDYNATTPL EPEVIQAVTE AMKEAWGNPS SSYVAGRKAK DIINTARASL AKMIGGKPQD IIFTSGGTES NNLVIHSTVR CFHEQQTLQG RTVDQISPEE GTRPHFITCT VEHDSIRLPL EHLVEDQVAE VTFVPVSKVN GQVEVEDILA AVRPTTCLVT IMLANNETGV IMPISEISRR IKALNQIRAA SGLPRVLVHT DAAQALGKRR VDVEDLGVDF LTIVGHKFYG PRIGALYVRG VGKLTPLYPM LFGGGQERNF RPGTENTPMI AGLGKAADLV SENCETYEAH MRDIRDYLEE RLEAEFGKRI HLNSRFPGVE RLPNTCNFSI QGSQLRGYMV LAQCQTLLAS VGASCHSDHE DRPSPVLLSC GIPVDVARNA VRLSVGRSTT RAEVDLIVQD LKQAVNQLEG PV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium.
Gene References into Functions
  1. ESI-MS analysis of wild-type and C375A mutant SCL revealed that the catalytic reaction proceeds via the formation of an enzyme-bound selenopersulfide intermediate on the catalytically essential Cys-375 residue. PMID: 20164179
  2. An enhancer element between bp -152 and -298 of the SCL 5'-regulatory region was identified, with protein binding to an AP-1 binding site that may be involved in the regulation of SCL-RNA in vivo in an endogenous feedback mechanism. PMID: 16874457
Subcellular Location
Cytoplasm, cytosol.
Protein Families
Class-V pyridoxal-phosphate-dependent aminotransferase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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