Recombinant Rat Sodium- and chloride-dependent glycine transporter 2 (Slc6a5), partial

Code CSB-YP021703RA
MSDS
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Source Yeast
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Code CSB-EP021703RA
MSDS
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Source E.coli
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Code CSB-EP021703RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021703RA
MSDS
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Source Baculovirus
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Code CSB-MP021703RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc6a5
Uniprot No.
Alternative Names
Slc6a5; Glyt2; Sodium- and chloride-dependent glycine transporter 2; GlyT-2; GlyT2; Solute carrier family 6 member 5
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sodium- and chloride-dependent glycine transporter. Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses.
Gene References into Functions
  1. Results show that glycine transport by neuronal glycine transporter GlyT2 responds positively to treatments promoting pain signaling including ATP. GlyT2 is up-regulated upon stimulation of ATP-preferring P2X2/3 receptors in brainstem/spinal cord primary neuronal cultures in a paracrine manner causing a rapid increase of surface transporter and glycine transport. PMID: 28734869
  2. Calnexin discriminates between different conformational states of GlyT2 displaying a lectin-independent chaperone activity. PMID: 23650557
  3. The clathrin-mediated pathway is the primary mechanism for constitutive and regulated GLYT2 endocytosis in heterologous cells and neurons. PMID: 21910806
  4. Using molecular interaction potential maps and mutagenesis, identified a conserved region in the GLYT2 external vestibule likely to be involved in Na+ interactions. Replacement of Asp471 reduced Na+ affinity and Na+ co-operativity of transport. PMID: 22132725
  5. In contrast to GLYT1, high and low affinity lithium-binding processes are detected in GLYT2. PMID: 21574997
  6. Both extracellular glycine and GlyT-2 are essential for forskolin-induced increase in amplitude of glycinergic miniature inhibitory postsynaptic currents during postnatal development. PMID: 20335821
  7. GLYT2 is associated with membrane rafts in the plasma membrane of brainstem terminals and neurons and is functionally modulated by the lipid environment. PMID: 18266927
  8. GLYT2 mRNA neurons are distributed in the ventrolateral medulla, the nucleus retroambiguus, the spinal trigeminal nucleus, the inferior olivary nucleus, and the border zone between the hypoglossal nucleus and reticular formation PMID: 14512139
  9. Activation of a Ca2+-dependent protease, likely calpain, in spinal cord synaptosomes or cultured spinal cord neurons, results in partial proteolysis; this may contribute to regulation of GlyT2 trafficking and/or function in the neuronal plasma membrane PMID: 14675166
  10. residue Lys-422 is crucial for glycine transport PMID: 15010455
  11. Many areas of the brainstem and cerebellum involve double-labeled neurons with GLYT2 and GAD67 mRNAs and suggests that the corelease of glycine and GABA from single neurons is more widespread than has been reported. PMID: 15140559
  12. Consecutive glycine uptake by GlyT2 and vesicular inhibitory amino acid transporter (VIAAT) is necessary and sufficient for concentrating glycine into vesicles. PMID: 17554001
  13. Data show the retina 2 expression of glycine transporter 1 in rats. PMID: 18695510
  14. study investigated the subcellular distribution of GLYT2 in brainstem tissue; data suggest that intracellular GLYT2 resides in a subset of endosomal membranes and may traffic around several compartments, mainly Rab11-positive endosomes PMID: 19374720
  15. Extracellular loops 2 and 4 of GLYT2 are required for N-arachidonylglycine inhibition of glycine transport. PMID: 19875446

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A5 subfamily
Tissue Specificity
Specifically expressed in spinal cord, brain stem, and to a lesser extent in the cerebellum.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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