Recombinant Rat Solute carrier family 22 member 8 (Slc22a8), partial

Code CSB-YP865648RA
MSDS
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Source Yeast
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Code CSB-EP865648RA
MSDS
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Source E.coli
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Code CSB-EP865648RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865648RA
MSDS
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Source Baculovirus
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Code CSB-MP865648RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc22a8
Uniprot No.
Alternative Names
Slc22a8; Oat3Solute carrier family 22 member 8; Organic anion transporter 3; rOat3
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Functions as one of the detoxification systems on the choroid plexus (CP) by removing substrates from the cerebrospinal fluid (CSF). Functions as an organic anion/dicarboxylate exchanger that couples organic anion uptake indirectly to the sodium gradient. Mediates the uptake from brain of organic anions, such as p-aminohippurate (PAH), ochratoxin A, and estrone sulfate (ES) and a cationic compound, cimetidine in a sodium independent manner. Mediates the uptake of benzylpenicillin (PCG), 2,4-dichloro-phenoxyacetate (2,4-D) and PAH in CP. Mediates the efflux of PAH, pravastatin, pitavastatin and PCG from the cerebrum into the blood circulation across the blood-brain barrier (BBB). Involved in regulating the CSF concentration of H(2)-receptor antagonists at the CP. Mediates the renal uptake of indoxyl sulfate (IS), 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) pravastatin, PCG, dehydroepiandrosterone sulfate (DHEAS) and is partly involved in the renal uptake of temocaprilat and ES.
Gene References into Functions
  1. Suggest OCT3 as possible norepinephrine uptake transporter in perivascular adipose tissue. PMID: 26432838
  2. The decreased function and expression of renal Oat3 in diabetes was associated with an increase in reactive oxygen species production coinciding with the activation of PKC and NF-kappaB signaling pathway. PMID: 23470271
  3. Gene and protein expressions of analyzed transporters increase as the brain matures, with no variation in their activity for P-gp and bcrp, while the transport activity of oat3 and oatp1a4 increases during brain maturation from preterm up to adulthood. PMID: 22998451
  4. Role of aromatic transmembrane residues in substrate recognition PMID: 12102636
  5. Organic anion transporter 3 functions as an organic anion/dicarboxylate exchanger that couples organic anion uptake indirectly to the Na(+) gradient. PMID: 12488248
  6. Cerebellar granule neurons accumulate MPP+ in manner that is consistent with uptake via OCT3 and the presence of this protein in CGNs explains their sensitivity to MPP+ toxicity PMID: 12675912
  7. rOAT3 is expressed at the abluminal membrane of the rat blood brain barrier and is involved in the brain-to-blood transport of HVA. PMID: 12679720
  8. Less pronounced regulation was detected for OCT2 and oatp3. Oatp1-3 and OCT2 may be important for endocrine function of rat adrenocortical cells. PMID: 12960058
  9. rOat1/hOAT1 and rOat3/hOAT3 play major roles in the renal uptake of uremic toxins on the basolateral membrane of the proximal tubules PMID: 14675047
  10. Rat OAT3 is involved in the brain-to-blood transport of thiopurines at the BBB and is one mechanism of limited cerebral distribution PMID: 15287899
  11. androgen receptor is involved in the functional regulation of OAT3 at the blood-brain barrier PMID: 15795901
  12. OAT3 abundance in both kidney cortex homogenates and in basolateral membranes increased by 3 days after bile duct ligation. PMID: 16844357
  13. rOat3 is responsible for the majority of rosuvastatin uptake across the basolateral membrane in rat kidney PMID: 17585018
  14. Oat3 can function in GSH uptake and that NRK-52E cells possess a low background rate of GSH uptake, making these cells a good model for overexpression of specific, putative GSH carriers PMID: 17719021
  15. OAT3 is responsible for renal secretion of bile acids during cholestasis and that the pharmacokinetic profile of OAT3 substrates may be affected by cholestasis. PMID: 18480179

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Subcellular Location
Basolateral cell membrane; Multi-pass membrane protein.
Protein Families
Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family
Tissue Specificity
Expressed in the liver, brain, kidney, choroid plexus and eye. Moderately expressed in the brain capillary endothelial cells (BCEC).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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