Recombinant Reston ebolavirus Minor nucleoprotein VP30 (VP30)

Code CSB-YP818305RCJ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP818305RCJ
MSDS
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Source E.coli
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Code CSB-EP818305RCJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818305RCJ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP818305RCJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VP30
Uniprot No.
Alternative Names
VP30; Minor nucleoprotein VP30; Transcription activator VP30
Species
Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus)
Expression Region
1-287
Target Protein Sequence
MEHSRERGRS SNMRHNSREP YENPSRSRSL SRDPNQVDRR QPRSASQIRV PNLFHRKKTD ALIVPPAPKD ICPTLKKGFL CDSKFCKKDH QLDSLNDHEL LLLIARRTCG IIESNSQITS PKDMRLANPT AEDFSQGNSP KLTLAVLLQI AEHWATRDLR QIEDSKLRAL LTLCAVLTRK FSKSQLGLLC ETHLRHEGLG QDQADSVLEV YQRLHSDKGG NFEAALWQQW DRQSLIMFIS AFLNIALQIP CESSSVVVSG LATLYPAQDN STPSEATNDT TWSSTVE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Multifunctional protein that acts as a viral transcriptional activator. Promotes read-through of an RNA hairpin in the NP open reading frame to enhance viral transcription. Mechanistically, nonphosphorylated VP30 hexamers form a ternary complex with the viral leader RNA. Clamps the RNA template and the complex VP35-polymerase L together, thereby increasing the polymerase affinity for the RNA template to increase transcription initiation despite the presence of RNA secondary structures. Assists also stop-start transcription at gene junctions to promote transcription of downstream genes. Interaction with NP plays a critical role in transcription initiation by recognizing the RNA stem loop. Interaction with host RBBP6 interferes with NP-VP30 interaction and inhibits viral RNA synthesis. Also acts as a suppressor of RNA silencing by interacting with host DICER1 and TARBP2/TRBP.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Filoviridae minor nucleoprotein VP30 family
Database Links

KEGG: vg:955196

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