Product Details

Purity

>85% (SDS-PAGE)

Target Names

MPH1

Uniprot No.

P40562

Alternative Names

MPH1; YIR002C; YIB2C; ATP-dependent DNA helicase MPH1; EC 3.6.4.12; FANCM-like protein 1; Mutator phenotype protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair. Prevents crossovers between ectopic sequences by removing substrates for MUS81-MMS4 or RAD1-RAD10 cleavage. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork (Probable). Binds to flap-structured DNA but not to non-flap nicked DNA, and participates in Okazaki fragment processing by stimulating the endonuclease activities of FEN1 and DNA2. Involved in recombination donor preference during mating-type switching via interaction with FKH1.

Gene References into Functions

Mutator phenotype of MPH1 overexpression suggests a novel gain-of-function mechanism. DEAH-box function was required for hypermutation, mutagenesis by MPH1 overexpression was independent of translesion synthesis, but was suppressed by overexpression of RAD27. PMID: 27585847
Mph1regulates the REC-mediated choice between gene conversion and break-induced replication (BIR). Double deletion of Sgs1 and Mph1 abolishes the REC-mediated lag observed during the repair of large gaps and BIR substrates. PMID: 27075725
Mte1 functions with Mph1 and the associated MHF complex in replication fork repair PMID: 26966246
Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres PMID: 26966248
Data support the hypothesis that Mph1 is the main helicase responsible for template switching during break-induced replication. PMID: 24496010
Mph1 does not affect the overall efficiency of gap repair, and its loss alters the CO-NCO by promoting synthesis-dependent-strand-annealing at the expense of HJ-containing intermediates. PMID: 23516370
Mph1 is a double-edge sword at the telomere, it prevents NHEJ, but promotes senescence in cells with dysfunctional telomeres by increasing the levels of ssDNA PMID: 22848695
Characterization of Mph1 helicase activity with a variety of DNA substrates, focusing on its action on junction structures containing three or four DNA strands. PMID: 22090425
These biochemical results not only provide validation for a role of Mph1 in the repair of damaged replication forks, but they also offer mechanistic insights as to its ability to efficiently disrupt the D-loop intermediate. PMID: 21880555
Mph1 has an additional function in recombination besides its previously described ability to disrupt D-loops; Mph1 promotes D-loop formation. PMID: 20951099
This study describes a synthetic growth defect of an mph1 deletion if combined with an srs2 deletion that can result-depending on the genetic background-in synthetic lethality. PMID: 19918932
the MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase PMID: 15634678
Mph1p promotes gross chromosomal rearrangement formation by partially suppressing homologous recombination, likely through its interaction with replication protein A. PMID: 18591428
a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks PMID: 19136626
These findings suggest that Mph1 plays an important, although not essential, role in processing of Okazaki fragments by facilitating the formation of ligatable nicks. PMID: 19181670

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Subcellular Location

Nucleus.

Protein Families

DEAD box helicase family, DEAH subfamily, FANCM sub-subfamily

Database Links

KEGG: sce:YIR002C

STRING: 4932.YIR002C

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Recombinant Saccharomyces cerevisiae ATP-dependent DNA helicase MPH1 (MPH1), partial

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