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Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor LTE1 (LTE1), partial

Recombinant Saccharomyces cerevisiae Guanine nucleotide exchange factor LTE1 (LTE1), partial

Code	CSB-YP362252SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP362252SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP362252SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP362252SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP362252SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

LTE1

Uniprot No.

P07866

Alternative Names

LTE1; EIS4; MSI2; YAL024CGuanine nucleotide exchange factor LTE1; Low temperature essential protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.; Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

Gene References into Functions

Data show that bud-localized MEN regulator Lte1 prevents Kin4 that escapes into the bud from associating with SPBs in the daughter cell. PMID: 21709215
Data suggest that Lte1 promotes mitotic exit by inhibiting Kin4 activity at the daughter spindle pole body. PMID: 21670215
Phosphorylation of Lte1 by Cdk prevents polarized growth during mitotic arrest in S. cerevisiae PMID: 21149565
Our findings suggest that Cla4-dependent phosphorylation promotes the initial association of Lte1 with Ras at the bud cortex and that Ras is required to stabilize phosphorylated forms of Lte1 at the bud cortex. PMID: 15917658
analysis of a link between the mitotic regulator, Lte1p, and protein processing and trafficking in the secretory/endosomal system PMID: 17121813
In lteDelta cells Brn1 release was delayed, even at temperatures that do not impair mitotic exit. PMID: 19387493
Lte1 can determine the subcellular localization of Bfa1 at spindle pole bodies (SPB) by promoting the loss of Bfa1 from the maternal SPB. PMID: 19948498