Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LTE1 Polyclonal antibody

Uniprot No.

P07866

Target Names

LTE1

Alternative Names

LTE1 antibody; EIS4 antibody; MSI2 antibody; YAL024CGuanine nucleotide exchange factor LTE1 antibody; Low temperature essential protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) LTE1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

GDP-GTP exchange factor for TEM1, a Ras-like protein, component of the mitotic exit network (MEN). Activation of TEM1 by LTE1 in the bud ultimately leads to activation of CDC15 followed by the release of CDC14 from the nucleolus, which then inactivates cyclin-dependent kinases (CDKs) activity by several mechanism. Required for TEM1 localization to the bud cortex during mitotic exit. Fine-tunes the timing of the mitotic exit and couples this event with cytokinesis.; Involved in proprotein-processing like proalpha factor-processing in the secretory pathway.

Gene References into Functions

Data show that bud-localized MEN regulator Lte1 prevents Kin4 that escapes into the bud from associating with SPBs in the daughter cell. PMID: 21709215
Data suggest that Lte1 promotes mitotic exit by inhibiting Kin4 activity at the daughter spindle pole body. PMID: 21670215
Phosphorylation of Lte1 by Cdk prevents polarized growth during mitotic arrest in S. cerevisiae PMID: 21149565
Our findings suggest that Cla4-dependent phosphorylation promotes the initial association of Lte1 with Ras at the bud cortex and that Ras is required to stabilize phosphorylated forms of Lte1 at the bud cortex. PMID: 15917658
analysis of a link between the mitotic regulator, Lte1p, and protein processing and trafficking in the secretory/endosomal system PMID: 17121813
In lteDelta cells Brn1 release was delayed, even at temperatures that do not impair mitotic exit. PMID: 19387493
Lte1 can determine the subcellular localization of Bfa1 at spindle pole bodies (SPB) by promoting the loss of Bfa1 from the maternal SPB. PMID: 19948498

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Subcellular Location

Cytoplasm. Bud. Note=The localization to the bud requires interaction with KEL1, as well as the presence of septins, CDC42, CLA4 and RAS2.

Protein Families

LTE1 family

Database Links

KEGG: sce:YAL024C

STRING: 4932.YAL024C

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LTE1 Antibody

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Target Background