Recombinant Saccharomyces cerevisiae Ribonucleoside-diphosphate reductase large chain 1(RNR1) ,partial

Code CSB-YP321975SVG
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Source Yeast
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Code CSB-EP321975SVG
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Source E.coli
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Code CSB-EP321975SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321975SVG
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Source Baculovirus
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Code CSB-MP321975SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names RNR1
Uniprot No. P21524
Alternative Names RNR1; CRT7; RIR1; SDS12; YER070W; Ribonucleoside-diphosphate reductase large chain 1; EC 1.17.4.1; Ribonucleotide reductase R1 subunit 1; Ribonucleotide reductase large subunit 1
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Gene References into Functions
  1. there is a directed orientation of the RNR beta and beta' C-terminal tails relative to alpha within the holoenzyme consistent with a docking model of the two subunits and argue against RT across the beta beta' interface PMID: 23532842
  2. The data reveaedl that the arginine 293 and glutamine 288 residues of ribonucleotide reductase 1 are crucial in facilitating ADP and CDP substrate selection. PMID: 22465672
  3. defects in autophagy result in an increase in soluble Rnr1 protein levels and a DNA damage phenotype. PMID: 21343333
  4. Data show that Rnr1 transcription requires protein bound to a particular site and either five different sites or one additional sites. PMID: 21103376
  5. The structures of the apo-, AMPPNP only-, AMPPNP-CDP-, AMPPNP-UDP-, dGTP-ADP- and TTP-GDP-bound complexes give insight into substrate and effector binding and specificity cross-talk. PMID: 16537479
  6. x-ray structures upon binding to gemcitabine diphosphate PMID: 16537480

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Subcellular Location Cytoplasm
Protein Families Ribonucleoside diphosphate reductase large chain family
Database Links

KEGG: sce:YER070W

STRING: 4932.YER070W

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