Recombinant Saccharomyces cerevisiae Tethering factor for nuclear proteasome STS1 (STS1)

Code CSB-YP336472SVG
MSDS
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Source Yeast
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Code CSB-EP336472SVG
MSDS
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Source E.coli
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Code CSB-EP336472SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336472SVG
MSDS
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Source Baculovirus
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Code CSB-MP336472SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STS1
Uniprot No.
Alternative Names
STS1; DBF8; SSM5; YIR011C; YIB11C; Tethering factor for nuclear proteasome STS1; Dumbbell former protein 8; SEC23 suppressor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-319
Target Protein Sequence
MMGFEWGFKP SSKITQSTVS SQGTGNVMIP TAGVKQKRRY ANEEQEEEEL PRNKNVMKYG GVSKRRPQPG SLIRGQPLPL QRGMELMNKN QLQQLLVDLM TKHPEIQQSV HTRVIGLDFS IQKCLDMLKQ KSEAVYQSIP YNRSYESNKL DDYAFVRMKP QILEFLNCLV DFILDNIPPR LENLHASLKF LDICTELVIK LPRFELASNN YYYDKCIEQL SHVWCTLIEH VARDRIILLA DNSSVWKSHM TRLQVYNEHS NGLLERPLQL FKSLDMGSPS AASSSTLSLQ ESIIYHHDTM TANENNNNSG SAATDSPFN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins. Required for efficient chromosome segregation. Restores protein transport and ribosomal RNA stability.
Gene References into Functions
  1. This study unveils a previously unknown role for Srp1 and Sts1 in cotranslational protein degradation and suggests a novel model whereby Srp1 and Sts1 cooperate to couple proteasomes to ribosome-bound nascent polypeptides. PMID: 24338021
  2. Sts1 plays a key role in targeting proteasomes to the nucleus. PMID: 21075847
  3. Sts1 is a new regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. PMID: 17916559
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Cut8/STS1 family
Database Links

KEGG: sce:YIR011C

STRING: 4932.YIR011C

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