Product Details

Purity

>85% (SDS-PAGE)

Target Names

sopB

Uniprot No.

O30916

Alternative Names

sopB; sigD; STM1091; Inositol phosphate phosphatase SopB; EC 3.1.3.-; Effector protein SopB

Species

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Converts phosphatidylinositol 3,4,5-trisphosphate (PtdIns 3,4,5-P3) to PtdIns 3-P and prevents the transition of PtdIns 3-P to PtdIns 3,5-P2. It is one of the known effectors injected by Salmonella into the host cell and is required for invasion and for an efficient generation and maintenance of Salmonella-containing vacuole (SVC). Alteration of the phosphoinositide composition of the plasma membrane causes membrane ruffling and actin cytoskeleton rearrangements. The persistence of PtdIns 3-P diverts the SCV from the endocytic pathway resulting in enlarged vesicles, which are essential to create a favorable environment where Salmonella can replicate and avoid immune defenses of the host cell.

Gene References into Functions

These findings show that SopB suppresses host cell apoptosis by binding to TRAF6 and preventing mitochondrial reactive oxygen species generation. PMID: 27473656
This work reveals the unique ability of bacterial effector protein SopB to utilize both its 4- and 5-phosphatase activities to regulate phosphoinositide dynamics to promote bacterial entry PMID: 26537021
SopB secretion was also considerably impaired in the absence of dam. PMID: 24947562
The structural organization of the N-terminus domain of SopB, a virulence factor of Salmonella, depends on the nature of its protein partners PMID: 24075929
Data found that SopB-dependent membrane translocation and phosphorylation of Akt are insensitive to the PI3K inhibitor wortmannin. PMID: 21779406
Killing of Candida albicans filaments by Salmonella enterica serovar Typhimurium is mediated by sopB effectors, parts of a type III secretion system PMID: 21498643
SopB may play a role in protecting mice from Salmonella colonization through activation of Akt2 PMID: 21555401
SopB activity results in dissociation of a number of host-cell endocytic trafficking proteins from this compartment and inhibits Salmonella-containing vacuole-lysosome fusion. PMID: 20542249
These data show that ubiquitination of SopB is not related to its intracellular stability but rather regulates its enzymatic activity at the plasma membrane and intracellular localization. PMID: 19614667
essential for the establishment of Salmonella's intracellular replicative niche; responsible for maintaining high levels of phosphatidylinositol-three-phosphate in the membrane of the bacteria-containing vacuoles PMID: 15205533
the N-terminal non-catalytic region exerts a specific effect on the actin cytoskeleton both in yeast and in human cell lines PMID: 16153243
study demonstrates that SopB, SopE, SopE2 and SipA are the Salmonella pathogenicity island-1 secreted effectors responsible for disruption of tight junction structure and function PMID: 16869830
Together, these results indicate that SPI-1 effector proteins SipA, SopA, SopB, SopD and SopE2 may participate in the late stages of Salmonella infection in mice. PMID: 17379731
When expressed by microinjecting cDNA into epithelial cells forming confluent monolayers, wild-type SigD induced striking morphological and functional changes that were not mimicked by a phosphatase-deficient SigD mutant PMID: 17389247
Study reports that the phosphoinositide phosphatase SopB, a Salmonella Typhimurium type III secreted effector protein, diversifies its function by localizing to different cellular compartments in a ubiquitin-dependent manner. PMID: 19379694
S. Typhimurium stimulates intestinal inflammationby delivering through its type III secretion system the bacterial effector proteins SopE, SopE2, and SopB PMID: 19662166
Prevents apoptosis in infected epithelial cells by activating the pro-survival kinase Akt. PMID: 15642738

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Subcellular Location

Secreted.

Protein Families

Phosphatase IpgD/SopB family

Database Links

KEGG: stm:STM1091

STRING: 99287.STM1091

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Recombinant Salmonella typhimurium Inositol phosphate phosphatase sopB (sopB), partial

Product Details

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Target Background

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