Home
Protein
Recombinant Protein
Recombinant Sheep Prion-like protein doppel (PRND)

Recombinant Sheep Prion-like protein doppel (PRND)

Code	CSB-YP880883SH
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP880883SH
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP880883SH-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP880883SH
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP880883SH
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

PRND

Uniprot No.

Q9GJY2

Alternative Names

PRND; Prion-like protein doppel; PrPLP

Species

Ovis aries (Sheep)

Expression Region

27-154

Target Protein Sequence

RGIK HRIKWNRKVL PSTSQVTEAH TAEIRPGAFI KQGRKLDINF GVEGNRYYEA NYWQFPDGIH YNGCSEANVT KEKFVTSCIN ATQVANQEEL SREKQDNKLY QRVLWQLIRE LCSIKHCDFW LERG

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Required for normal acrosome reaction and for normal male fertility. Can bind Cu(2+).

Gene References into Functions

the results of the present study confirm that the identification of different PRND genotypes is important for studying the sperm capacitation process and for improving sperm cryoresistance and embryo production. Furthermore, the detection of Doppel in ejaculated ovine spermatozoa, along with its low expression after cryopreservation, strongly suggests an important physiological function of this protein in male fertility. PMID: 28442049
A novel 20-bp indel within sheep PRND gene was verified in 623 tested individuals representing 4 diversity sheep breeds. PMID: 28362554
Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability. PMID: 21806689
These results constitute preliminary evidence of an association between a polymorphism in the PRND gene and scrapie susceptibility PMID: 19968641
Dpl is enriched in residual bodies, which are phagocytosed and destroyed by Sertoli cells after release of sperm into the lumen of the seminiferous tubule PMID: 16390495

Hide All

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Prion family

Tissue Specificity

Strongly expressed in testis. Detected at low levels in lymph node, spleen and ovary.

Database Links

KEGG: oas:443194

UniGene: Oar.838

Promotions