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Recombinant Thermus thermophilus SsrA-binding protein (smpB)

Recombinant Thermus thermophilus SsrA-binding protein (smpB)

Code	CSB-YP823302TNT
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP823302TNT
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP823302TNT-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP823302TNT
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP823302TNT
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

smpB

Uniprot No.

Q8RR57

Alternative Names

smpB; TTHA0902; SsrA-binding protein; Small protein B

Species

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Expression Region

1-144

Target Protein Sequence

MAPVLENRRA RHDYEILETY EAGIALKGTE VKSLRAGKVD FTGSFARFED GELYLENLYI APYEKGSYAN VDPRRKRKLL LHKHELRRLL GKVEQKGLTL VPLKIYFNER GYAKVLLGLA RGKKAYEKRR EDKKEAVRRA LEEL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for rescue of stalled ribosomes mediated by trans-translation. Binds to tmRNA (also known as SsrA and 10Sa), required for stable association of tmRNA with ribosomes, probably by bridging tmRNA to 50S subunit. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.

Gene References into Functions

crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 A; structure shows how SmpB plays role of both anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon PMID: 22422985
SmpB undergoes structural changes indicating an induced fit upon binding to the specific region of tRNA-like domain of tmRNA. PMID: 16428302

Subcellular Location

Cytoplasm.

Protein Families

SmpB family

Database Links

KEGG: ttj:TTHA0902

STRING: 300852.TTHA0902

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