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Recombinant Vaccinia virus Protein A36 (VACWR159), partial

Recombinant Vaccinia virus Protein A36 (VACWR159), partial

Code	CSB-YP303745VAI
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP303745VAI
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP303745VAI-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP303745VAI
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP303745VAI
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

VACWR159

Uniprot No.

P68619

Alternative Names

VACWR159; A36RProtein A36

Species

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Involved in the intracellular transport of virions to the host cell surface. Participates also in the formation of actin tails at the plasma membrane to allow efficient actin-based motility and thus cell to cell transmission of viral particles. Recruits host intersectin-1/ITSN1 and activates host CDC42 to drive ARP2/3-mediated actin polymerization.

Gene References into Functions

study reports that A36 contains three NPF motifs that interact with the Eps15 homology (EH) domains in intersectin-1 and Eps15 to promote the release of virus from infected cells and their subsequent spread PMID: 27670116
janosi PMID: 22980329
The authors demonstrate that clathrin promotes clustering of the vaccinia virus actin tail nucleator A36 and host N-WASP, which activates actin nucleation through the Arp2/3 complex. PMID: 22980331
findings show that a bipartite tryptophan-based motif in A36 is required for kinesin-1-dependent transport of the virus to the cell periphery. PMID: 21915095
we show that F12 has structural similarity to kinesin light chain PMID: 20195521

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Subcellular Location

Host cell membrane; Single-pass membrane protein. Note=Found exclusively on the wrapped enveloped virion. Absent in the mature virion (MV) and extracellular enveloped virion (EV).

Protein Families

Orthopoxvirus A36 protein family

Database Links

KEGG: vg:3707689

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