Recombinant Vaccinia virus Protein A46 (VACWR172)

Code CSB-YP326310VAI
MSDS
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Source Yeast
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Code CSB-EP326310VAI
MSDS
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Source E.coli
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Code CSB-EP326310VAI-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326310VAI
MSDS
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Source Baculovirus
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Code CSB-MP326310VAI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VACWR172
Uniprot No.
Alternative Names
VACWR172; A46RProtein A46
Species
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Expression Region
1-240
Target Protein Sequence
MAFDISVNAS KTINALVYFS TQQNKLVIRN EVNDTHYTVE FDRDKVVDTF ISYNRHNDTI EIRGVLPEET NIGCAVNTPV SMTYLYNKYS FKLILAEYIR HRNTISGNIY SALMTLDDLA IKQYGDIDLL FNEKLKVDSD SGLFDFVNFV KDMICCDSRI VVALSSLVSK HWELTNKKYR CMALAEHISD SIPISELSRL RYNLCKYLRG HTESIEDKFD YFEDDDSSTC SAVTDRETDV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
BCL2-like protein which disrupts the host immune response by inhibiting the TLR4 signaling pathway leading to NF-kappa-B activation. Acts close to the plasma membrane and targets several host TIR-domain containing adaptor proteins including MYD88, TIRAP, TRIF and TICAM2. In turn, blocks the host NF-kappa-B and TRIF-mediated IRF3 activation.
Gene References into Functions
  1. Results show that A46 is a homodimer with a Bcl-2-like fold and VIPER motif is believed to be involved in the interaction with MAL protein. PMID: 24723367
  2. Characterization and structure of the vaccinia virus NF-kappaB antagonist A46. PMID: 24356965
  3. The region of A46 from which VIPER is derived represents the TLR4-specific inhibitory motif of the intact protein, and is essential for A46:TRAM interactions. PMID: 22593572
Protein Families
Poxviridae A46R protein family
Database Links

KEGG: vg:3707702

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