Recombinant Xenopus laevis ATP-dependent RNA helicase DDX25 (deadsouth)

Code CSB-YP887618XBE
MSDS
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Source Yeast
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Code CSB-EP887618XBE
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Source E.coli
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Code CSB-EP887618XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887618XBE
MSDS
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Source Baculovirus
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Code CSB-MP887618XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
deadsouth
Uniprot No.
Alternative Names
deadsouthATP-dependent RNA helicase DDX25; EC 3.6.4.13; DEAD box protein 25; RNA helicase DEADSouth; Xcat3
Species
Xenopus laevis (African clawed frog)
Expression Region
1-483
Target Protein Sequence
MAAKFLPRFW RSGSQAELLD FQNNNVVAEG KLDFEHGTLK GKSGRYGDDE EDVRRGHIED LANHSLLNKL LRRTLVDSPH NVEVLQRDPT SPLFSVKSFE ELHLKNELLR GIYAMGFNRP SKIQENALPM MLADPPQNLI AQSQSGTGKT AAFVLAMLSR VDANKKYPQC ICLSPTFELA LQTGKVVEEM GKFCAGIEVI YALRGNRPGK GSRLEAQIVI GTPGTVLDWC FKLRLITVEN ISVFVLDEAD VMINVQGHSD HSVRVKRSMP KSCQMLLFSA TFEDSVWAFA ERIVPDPNII KLKKEELTLK NIQQFYDQCE NKEQKYSALC NLYGVITIAQ AIVFCQTRKI ASWLSQKLSD DGHQVALLSG ELPVYDRADM IQRFREGREK VLVTTNVCAR GIDVEQVSIV VNFDLPVNVD GSVDFETYLH RIGRTGRFGK KGIAVSLIEN FFVYMLKEIE DHFNTKITKL NSMDMDEMGK IWK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATP-dependent RNA helicase.
Gene References into Functions
  1. the predominant expression of DEADSouth mRNA in the PGCs is ensured by multiple mechanisms including zygotic expression and prohibition from miR-427-mediated degradation. PMID: 24291337
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
DEAD box helicase family
Tissue Specificity
An mRNA component of germ plasm. Localizes to the granulo-fibrillar material (GFM) of the mitochondrial cloud in stage I oocytes. Associated, at a low level, with the periphery of mature germinal granules in later stage oocytes. Localizes to the vegetal c
Database Links

KEGG: xla:398180

UniGene: Xl.670

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