Recombinant Xenopus laevis Cyclin-dependent kinase 1-A (cdk1-a)

Code CSB-YP336053XBE
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP336053XBE
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP336053XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP336053XBE
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP336053XBE
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
cdk1-a
Uniprot No.
Alternative Names
cdk1-a; cdc2-a; cdc2x1.1Cyclin-dependent kinase 1-A; CDK1-A; EC 2.7.11.22; EC 2.7.11.23; Cell division control protein 2 homolog 1; Cell division control protein 2-A; Cell division protein kinase 1-A; p34 protein kinase 1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-302
Target Protein Sequence
MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR EISLLKELQH PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI DTMLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDSKG VIKLADFGLA RAFGIPVRVY THEVVTLWYR APEVLLGSVR YSTPVDVWSI GTIFAEIATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNS FPKWKGGSLS ANVKNIDKDG LDLLAKMLIY DPAKRISARK ALLHPYFDDL DKSSLPDNQI RN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. May play a role in regulating the amplitude of the cyclic expression of circadian clock genes.
Gene References into Functions
  1. the fine-tuning of Cdc6 accumulation is essential to ensure two meiotic waves of Cdk1 activation and to avoid unscheduled DNA replication during meiotic maturation. PMID: 26092930
  2. equilibrium between CDK1 and PP2A specifies the timing of M-phase entry and exit and regulates the dynamics of cyclin B degradation upon M-phase exit in Xenopus laevis first embryonic mitosis. PMID: 26154318
  3. CDK1 activation proceeds with concomitant inhibition by CDC6, which tunes the timing of the M-phase entry during the embryonic cell cycle PMID: 25264619
  4. Xenopus Cdk1-AS rescues HT2-19 cells from apoptosis. PMID: 23525902
  5. Ras suppresses cyclin-dependent kinase 1 in a complex manner: It induces continuous accumulation of cyclin B2, but also causes persistent inhibitory phosphorylation of tyr-15-cyclin-dependent kinase 1. PMID: 23376039
  6. By promoting CtIP-dependent resection of double-strand break (DSB) ends while preventing Rad51 chromatin assembly, Cdk1 inhibits both the nonhomologous and homologous modes of DSB repair during mitosis. PMID: 21893598
  7. Examination of H1 histones reveals isoform-specific regulation by Cdk1 and RanGTP; mitotic Cdk1 functions to enhance H1 binding in egg extracts and embryos PMID: 20471264
  8. Cdc2 displays cytoskeleton-dependent localization in blastomere cortex during Xenopus embryonic cell cycle. PMID: 16097011
  9. Results suggest that the specific synthesis of either B-type cyclins or c-Mos, induced by progesterone, is required to induce meiotic maturation and Cdc2 activation. PMID: 16374506
  10. Cdk1/cyclin B has a role in regulating B-raf activation at mitosis PMID: 18434602

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
Database Links

KEGG: xla:380246

UniGene: Xl.8917

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1