Recombinant Xenopus laevis Desert hedgehog protein A (dhh-a)

Code CSB-YP820936XBE
MSDS
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Source Yeast
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Code CSB-EP820936XBE
MSDS
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Source E.coli
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Code CSB-EP820936XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP820936XBE
MSDS
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Source Baculovirus
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Code CSB-MP820936XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
dhh-a
Uniprot No.
Alternative Names
dhh-a; chhDesert hedgehog protein A; Cephalic hedgehog protein; Desert hedgehog protein 1; DHH-1; X-CHH) [Cleaved into: Desert hedgehog protein A N-product; Desert hedgehog protein A C-product]
Species
Xenopus laevis (African clawed frog)
Expression Region
23-396
Target Protein Sequence
CGPGRGPV GRRRYMRKLV PLHYKQFVPN VPEKTLGASG KSEGKIHRGS ERFIELVPNY NPDIIFKDEE KTGADRLMTE RCKDRVNALA ISVMNMWPGV KLRVTEGWDE DGHHAHDSLH YEGRALDITT SDRDRNKYGM LARLAVEAGF DWVYYESKAH IHVSVKADNS LGVRSGGCFP GTAMVMMGTG ERKPLSELKI GDTVYTTDET GQLITSVVLL FLHRNPYKTA TFVLIEAEGH PSKLLVTPNH LLFIQSSSSA GFLPTFAYRV QIGDLVQIYV NGTQVQSSKV VRVSLEEQTG VYAPMTEHGT LLVDGVLTSC YATVESHTLA HVSLAPLRLF QGIASMLPDL DMSDGVHWYC HILYVLAKYV LWWDMP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Signal involved in the early induction and patterning of anterodorsal ectoderm, nervous system and somites. Induces ectopic cement gland formation in embryos.
Subcellular Location
[Desert hedgehog protein A N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Desert hedgehog protein A C-product]: Secreted, extracellular space.
Protein Families
Hedgehog family
Tissue Specificity
Expressed in the marginal zone at early gastrulation. In the neurula, expression is restricted to anterior structures, encompassing both neural plate and endodermal cells. Expressed on the inner surface of the pharynx at the early tadpole stage.
Database Links

KEGG: xla:378538

UniGene: Xl.999

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