Recombinant Xenopus laevis T-box protein VegT-A (vegt-a)

Code CSB-YP309325XBE
MSDS
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Source Yeast
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Code CSB-EP309325XBE
MSDS
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Source E.coli
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Code CSB-EP309325XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP309325XBE
MSDS
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Source Baculovirus
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Code CSB-MP309325XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
vegt-a
Uniprot No.
Alternative Names
vegt-a; apod; brat; vegtT-box protein VegT-A; Brachyury and Tbx-related protein; T-box protein Brat; T-box protein Antipodean
Species
Xenopus laevis (African clawed frog)
Expression Region
1-455
Target Protein Sequence
MRNCCRECGL SAGHLEPEAS SNCASDVKSS PDMDSVSSQD SLYLPNTVGA SLEDQDLWSQ FHQEGTEMII TKSGRRMFPQ CKIRLFGLHP YAKYMLLVDF VPLDNFRYKW NKNQWEAAGK AEPHPPCRTY VHPDSPAPGA HWMKDPICFQ KLKLTNNTLD QQGHIILHSM HRYKPRFHVV QSDDMYNSPW GLVQVFSFPE TEFTSVTAYQ NEKITKLKIN HNPFAKGFRE QERSHKRDDV LKILQQSPSK RQKRKKWEDS PEADISDFPK AICVKEESIM DPAGVYQNWV SDHEANQGLT PHSPESEGAN QEQQVPTSSS NFYNKSHYRR SSQHLSSPFE LGEPSSRRLT PDIATVPDSD PDSLAVFHVI PTQNSAPERT CSMNFSMEAP MKQPLRGAMY SPYGADQWLV PAQGQYRPVG YTAYPTDLST QGAVAHPHSA MSDWSQYSLF PYSCW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor required for both mesoderm and endoderm formation in the embryo; signaling determinants and concentration levels may determine which germ layer is formed. Acts together with beta-catenin to activate genes that are responsible for mesoderm induction including wnt-8, eomes t/bra, siamois, mix1 and sox17. Directly binds to promoter DNA. Patterns the mesoderm along the dorsoventral and posterior axis. Activates siamois gene transcription when alone or in combination with beta-catenin, but inhibits siamois transcription in combination with pou5f1.1/oct-25.
Subcellular Location
Nucleus.
Tissue Specificity
Uniformly distributed in stage I oocytes but becomes localized to the vegetal hemisphere by stage II and remains so thereafter throughout oogenesis and the early embryonic cleavage stages. Zygotic expression parallels blastopore formation and shifts from
Database Links

KEGG: xla:397986

UniGene: Xl.1775

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