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Recombinant Xenopus laevis T-box transcription factor TBX6 (tbx6), partial

Recombinant Xenopus laevis T-box transcription factor TBX6 (tbx6), partial

Code	CSB-YP805208XBE
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP805208XBE
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP805208XBE-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP805208XBE
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP805208XBE
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

tbx6

Uniprot No.

Q8AV66

Alternative Names

tbx6; T-box transcription factor TBX6; T-box protein 6; XTbx6

Species

Xenopus laevis (African clawed frog)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Transcriptional activator. Plays a role in ventral mesoderm specification and acts together with nog during muscle differentiation. Mediates posterior pattern formation of the embryo, including specification of posterior mesoderm, via activation of the Wnt and Fgf signaling pathways.

Gene References into Functions

Tbx6 is required for neural crest and intermediate mesoderm development in Xenopus PMID: 20083100
several signaling pathways are involved in regulating Tbx6 expression in the initiation of myogenesis PMID: 15188053
FGF8, Wnt8 and Myf5 are target genes of Tbx6 during anteroposterior specification in Xenopus embryo PMID: 16343478
Data show that morpholino-induced knockdown of Xenopus Tbx6 impairs posterior development, indicating the requirement of XTbx6 in this process. PMID: 16953215
Based on our findings, we conclude that a part of the transcriptional repression at the anterior end of the PSM is caused by Bowline mediated transcriptional repression of Tbx6-dependent gene expression in X. laevis. PMID: 17577580
These results indicate that the appropriate expression of bowline/Ripply2 is regulated by a direct interaction between the Tbx6 and mespb proteins during Xenopus somitogenesis. PMID: 18510946
Xtbx6 is a direct regulator of pMesogenin1 and 2, which are both involved in somitogenesis and myogenesis including that of body wall muscle in Xenopus laevis PMID: 19035338

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Subcellular Location

Nucleus.

Tissue Specificity

At gastrulation, expressed in the lateral and ventral mesoderm. Expressed around the blastopore except at the dorsal midline. As gastrulation proceeds, expression extends anteriorly and ventrally. At the tailbud stage, expression then retracts caudally be

Database Links

KEGG: xla:394428

UniGene: Xl.7697

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