Recombinant Xenopus laevis Transcription factor Sox-17-alpha-A (sox17a-a)

Code CSB-YP667291XBE
MSDS
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Source Yeast
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Code CSB-EP667291XBE
MSDS
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Source E.coli
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Code CSB-EP667291XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP667291XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP667291XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sox17a-a
Uniprot No.
Alternative Names
sox17a-a; sox17a; sox17a1; Transcription factor Sox-17-alpha-A; Xsox17alpha; xSox17alpha1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-380
Target Protein Sequence
MSSPDGGYAS DDQNQGKCSV PIMMTGLGQC QWAEPMTSLG EGKLKSDAGS ANSRSKAEAR IRRPMNAFMV WAKDERKRLA QQNPDLHNAE LSKMLGKSWK SLTLAEKRPF VEEAERLRVQ HMQDHPNYKY RPRRRKQVKR MKRAENGFMH MAEAQESAVL GADGRMCLEN FSLGYHEQTY PHGQVPQSSH YREPQAVAPH YDGYSLPTPE SSPLDLAEAD PVFFTSPAQD ECQMMPYSYN GSYPHQQNSM LVRQMPQTEQ MGQVSPVQGM MACQSSPHMY YGQMYLPGSA RHHQLHQAGQ PSPPPEAQQM GRADHIQPAD MLAEVDRTEF EQYLSYVAKS DLGMHYHAQE SVVPTADNGP ISSVLSDAST AVYYCNYPSA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator. Binds to the DNA sequence 5'-AACAAT-3'. All of the sox17 proteins are required for embryonic endoderm development and gastrulation movements, and show some redundancy in function. In addition, the sox17 proteins have distinct but overlapping roles in later gut development. Acts downstream of vegt-signaling in endoderm differentiation to induce a range of endodermal genes both directly (including endodermin and dhh/chh) and indirectly. Also represses wnt-responsive genes to inhibit wnt/beta-catenin signaling.
Gene References into Functions
  1. two regions co-operate in regulating the early endodermal expression of the Xsox17alpha(1) gene PMID: 17719026
Subcellular Location
Nucleus.
Tissue Specificity
In early gastrulae, expressed in the vegetal but not animal hemisphere. The vegetal region is fated to become endoderm, and endodermal expression continues throughout gastrulation and neurulation. At tailbud stages, expression is down-regulated and become
Database Links

KEGG: xla:397966

UniGene: Xl.3831

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