Recombinant Xenopus tropicalis Serine/threonine-protein kinase NLK2 (nlk.2)

Code CSB-YP536620XBF
MSDS
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Source Yeast
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Code CSB-EP536620XBF
MSDS
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Source E.coli
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Code CSB-EP536620XBF-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP536620XBF
MSDS
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Source Baculovirus
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Code CSB-MP536620XBF
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nlk.2
Uniprot No.
Alternative Names
nlk.2; Serine/threonine-protein kinase NLK2; EC 2.7.11.24; Nemo-like kinase 2; Nlk.2
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region
1-454
Target Protein Sequence
MAFQGPGRSL PGQLCAGVFG GLIQPPLGQK FYCPNGGSGG GGVPAVPSPL PQALSAPQCN GDGRGEPEPD RPIGYGAFGV VWSVTDPRDG KRVALKKMPN VFQNLVSCKR VFRELKMLCF FKHDNVLSAL DILQPPQIDC FEEIYVITEL MQTDLHKVIV SPQPLSSDHI KVFLYQILRG LKYLHSAGIL HRDIKPGNLL VNSNCVLKIC DFGLARVEEL DESQHMTQEV VTQYYRAPEI LMGSRHYRSA IDIWSVGCIF AELLGRRILF QAQSPIQQLD LITDLLGTPP LTAMRSACEG ARAHILRGPH KPPSLSVLYM LSGEATHEAV HLLCRMLLFD PLKRISAKDA LAHPYLEEGR LRYHTCMCHC CYSVSSGRVY TADFEPTATN RFDDSYEKSL TSVWQVKELV HRFITDQQQG KRPPLCINPH SAAFKTFIRS TAWHSSKVSK KEER
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negatively regulates Wnt/beta-catenin-signaling during development. Plays a role together with sox11 in neural induction during early embryogenesis. Involved in TGFbeta-mediated mesoderm induction in early embryos, acting downstream of map3k7/tak1 to phosphorylate stat3. Augments the rnf138/narf-directed ubiquitination and degradation of tcf/lef by enhancing the association of rnf138/narf and tcf/lef. Phosphorylates mef2a to play a role in anterior neural development, including eye formation.
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

KEGG: xtr:100144684

UniGene: Str.64836

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