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| Code | CSB-EP015303HUe0 |
| Size | $1812 |
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| Description |
Recombinant Human Myosin-9/MYH9 produced in E.coli contains 240 amino acids covering the partial-length of MYH9 protein (2-241AA). And it has its N-terminus fused with a GST-tag and a total molecular weight of 54.2 kDa. The purity of this human MYH9 protein is greater than 90% measured by SDS-PAGE. This protein is in-stock, which means there is no waiting period for protein preparation. This recombinant MYH9 protein may find uses in the specific antibody synthesis or the studies of the cell cycle. MYH9, also called NMM-IIA, is a myosin that exerts a vital role in cell adhesion, migration, proliferation, and differentiation. Acting as a cytokine, MYH9 executes proliferative and progressive effects in cancer by participating in cytoskeletal reorganization, cellular pseudopodia formation, and migration. Additionally, MYH9 is closely associated with the progress and poor prognosis of most solid tumors. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | MYH9 |
| Uniprot No. | P35579 |
| Research Area | Cell Cycle |
| Alternative Names |
BDPLT 6; BDPLT6; Cellular myosin heavy chain; Cellular myosin heavy chain type A; DFNA 17; DFNA17; EPSTS; FTNS; MGC104539; MHA; MYH 2A; MYH 9; MYH2A; MYH9; MYH9_HUMAN; MYHas8; MyHC 2A; MyHC IIa; MyHC2A; MyHCIIa; MYHSA 2; MYHSA2; Myosin 9; Myosin heavy chain 9; Myosin heavy chain 9 non muscle; Myosin heavy chain; Myosin heavy chain non muscle IIa; Myosin heavy chain nonmuscle IIa; Myosin heavy polypeptide 2; Myosin heavy polypeptide 9 non muscle; Myosin-9; Myosin9; NMHC II A; NMMHC A; NMMHC II a; NMMHC II-a; NMMHC IIA; NMMHC-A; NMMHC-IIA; NMMHCA; Non muscle myosin heavy chain A; Non muscle myosin heavy chain; Non muscle myosin heavy chain II A; Non muscle myosin heavy polypeptide 9; non-muscle IIa; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; Nonmuscle myosin heavy chain A; Nonmuscle myosin heavy chain II A; type A
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| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 2-241aa |
| Target Protein Sequence | AQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRI Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 54.2kDa |
| Protein Length | Partial |
| Tag Info |
N-terminal GST-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction; this function is mechanically antagonized by MYH10.
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| Gene References into Functions |
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| Involvement in disease | May-Hegglin anomaly (MHA); Sebastian syndrome (SBS); Fechtner syndrome (FTNS); Epstein syndrome (EPSTNS); Deafness, autosomal dominant, 17 (DFNA17); Macrothrombocytopenia and progressive sensorineural deafness (MPSD) |
| Subcellular Location | Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cytoplasmic vesicle, secretory vesicle, Cortical granule. |
| Protein Families | TRAFAC class myosin-kinesin ATPase superfamily, Myosin family |
| Tissue Specificity | In the kidney, expressed in the glomeruli. Also expressed in leukocytes. |
| Database Links |
HGNC: 7579 OMIM: 153640 KEGG: hsa:4627 STRING: 9606.ENSP00000216181 UniGene: Hs.474751 |
