ATF5 Antibody

Code CSB-RA180846A0HU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue
    All lanes: ATF5 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 31 kDa
    Observed band size: 35 kDa
  • IHC image of CSB-RA180846A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No. Q9Y2D1
Target Names ATF5
Alternative Names Cyclic AMP-dependent transcription factor ATF-5 (cAMP-dependent transcription factor ATF-5) (Activating transcription factor 5) (Transcription factor ATFx), ATF5, ATFX
Species Reactivity Human, Rat
Immunogen A synthesized peptide derived from human ATF5
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 9D8
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus
Gene References into Functions
  1. Data suggest that ATF5 is modified by SUMO2/3 at a conserved SUMO-targeting consensus site; this SUMOylation of ATF5 appears to be required for transport of ATF5 to centrosome. (ATF5 = activating transcription factor-5; SUMO = small ubiquitin-like modifier) PMID: 29326161
  2. Study reports that reduced levels of ATF5 in brain of Huntington's disease patients, probably due to its sequestration into the characteristic PolyQ containing neuronal inclusion bodies, correlates with decreased levels of the antiapoptotic protein MCL1, a transcriptional target of ATF5. Also provides evidence of decreased ATF5 being deleterious by rendering neurons more vulnerable to polyQ-induced apoptosis. PMID: 28861715
  3. ATF5 expression can rescue UPR(mt) signaling in atfs-1-deficient worms requiring the same UPR(mt) promoter element identified in C. elegans. Furthermore, mammalian cells require ATF5 to maintain mitochondrial activity during mitochondrial stress and promote organelle recovery. Combined, these data suggest that regulation of the UPR(mt) is conserved from worms to mammals. PMID: 27426517
  4. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-alpha2 and integrin-beta1 in several human cancer cell lines. PMID: 27125458
  5. This study provides the first evidence that the methylation level of ATF5 decreased, and its mRNA expression was evidently up-regulated in glioma. PMID: 26365117
  6. These results suggest that the hepatic functions of the human iPS-HLCs could be enhanced by ATF5, c/EBPalpha, and PROX1 transduction. PMID: 26679606
  7. Activating transcription factor 5 enhances radioresistance and malignancy in cancer. PMID: 25682872
  8. Data show that ATF5 is an essential structural protein that is required for the interaction between the mother centriole and the pericentriolar material. PMID: 26213385
  9. Low expression level of ATF5 in hepatocellular carcinoma indicated aggressive tumor behavior and predicted a worse clinical outcome. PMID: 25294425
  10. Report a global loss of 5hmC identified three new genes (ECM1, ATF5, and EOMES) with potential anti-cancer functions that may promote the understanding of the molecular mechanisms of hepatocellular carcinoma development and progression. PMID: 25517360
  11. the TAK1-NLK pathway is a novel regulator of basal or IL-1beta-triggered C/EBP activation though stabilization of ATF5 PMID: 25512613
  12. ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection PMID: 24302293
  13. N-terminal hydrophobic amino acids play an important role in the regulation of ATF5 protein expression in IL-1beta-mediated immune response and that ATF5 is a negative regulator for IL-1beta-induced expression of SAA1 and SAA2 in HepG2 cells. PMID: 24379400
  14. The 5'-untranslated region regulates ATF5 mRNA stability via nonsense-mediated mRNA decay in response to environmental stress. PMID: 23876217
  15. demonstrated that interference with the function of ATF5 could markedly increase the apoptosis of ovarian cancer cells and identified B-cell leukemia lymphoma-2 as an ATF5-targeted apoptosis-related gene PMID: 23018213
  16. The evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose-derived stem cells. PMID: 22442021
  17. a mechanistic link between elevated NPM1 expression and depressed ATF5 in HCC and suggests that regulation of ATF5 by NPM1 plays an important role in the proliferation and survival of HCC. PMID: 22528486
  18. ATF5 polymorphisms influence ATF function and response to treatment in children with childhood acute lymphoblastic leukemia. PMID: 21972289
  19. coordinated actions by ATF5, p300, Elk-1, and ERK/mitogen-activated protein kinase are essential for ATF5-dependent Egr-1 activation and cell proliferation and survival PMID: 21791614
  20. an essential role for HSP70 in maintaining high levels of ATF5 expression in glioma cells and support the conclusion that ATF5 is an important substrate protein of HSP70 that mediates HSP70-promoted cell survival in glioma cells. PMID: 21521685
  21. BCL-2 is an essential mediator for the cancer-specific cell survival function of ATF5 in glioblastoma and breast cancer cells PMID: 21212266
  22. our studies not only provided molecular basis of ATF5 transcriptional regulation, but also identified ATF5 as a target gene of EBF1 transcription factor. PMID: 20423929
  23. activating transcription factor 5 (ATF5) is a new interacting partner of cyclin D3 PMID: 15358120
  24. recruitment of ATFx to the HTLV-1 LTR serves to link viral transcription with critical events in cellular homeostasis PMID: 15890932
  25. Overexpression of the bZIP protein ATF5, a transcriptional activator, stimulates asparagine synthetase promoter/reporter gene transcription via the nutrient-sensing response unit. PMID: 16164412
  26. The widespread expression of ATF5 in glioblastomas PMID: 16170340
  27. ATF5 increases cisplatin-induced apoptosis through up-regulation of Cyclin D3 transcription, which elicits survival signals in HeLa cells PMID: 16300731
  28. Contribution of common variations of ATF4 and ATF5 to the pathophysiology of bipolar disorder may be minimal if any. PMID: 17346882
  29. translation of ATF5 is regulated by the alternative 5'-UTR region of its mRNA, and ATF5 may play a role in protecting cells from amino acid limitation or arsenite-induced oxidative stress PMID: 18055463
  30. ATF4 contributes to basal ATF5 transcription, and eIF2 kinases direct the translational expression of multiple transcription regulators by a mechanism involving delayed translation reinitiation PMID: 18195013
  31. ATF5 is abundant in the liver, activates CYP2B6, and cooperates with the constitutive androstane receptor in sustaining the hepatic-specific expression of this P450 in human hepatocytes and hepatoma cells. PMID: 18332083
  32. cisplatin increased ATF5 protein expression via preventing its ubiquitin-dependent degradation, which might be associated with its promoting the nucleus-to-cytoplasm translocation of E2 ubiquitin-conjugating enzyme Cdc34 PMID: 18458088
  33. Loss of ATF5 is associated with hepatocellular carcinoma PMID: 18701499
  34. The down-regulation of the SAP gene by ATF5 may represent a common mechanism for the pathogenesis of Hemophagocytic syndrome that is associated with either Epstein-Barr virus infection or immune disorders with dysregulated T-cell activation. PMID: 18832568
  35. These results indicate that ATF5 is targeted for degradation by the ubiquitin-proteasome pathway, and that cadmium slows the rate of ATF5 degradation via a post-ubiquitination mechanism. PMID: 19285020
  36. Identified a novel ATF5 consensus DNA binding sequence. Demonstrate in C6 glioma and MCF-7 breast cancer cells that ATF5 occupies this sequence and that ATF5 activates reporter gene expression driven by this site. PMID: 19531563

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Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Protein Families BZIP family
Tissue Specificity Widely expressed with higher expression levels in liver.
Database Links

HGNC: 790

OMIM: 606398

KEGG: hsa:22809

STRING: 9606.ENSP00000396954

UniGene: Hs.9754

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