Code | CSB-RA180846A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The ATF5 recombinant antibody is prepared using protein and DNA recombinant technology. To produce the antibody, mice were first immunized with a synthetic peptide derived from human ATF5. After a certain period of time, spleen cells were collected under aseptic conditions, and total RNA was extracted from spleen cells and then converted into cDNA synthesized through RNA reverse transcription. The cDNA was used as a template for PCR amplification of the ATF5 antibody gene. The resulting ATF5 antibody gene was then introduced into a vector and transfected into host cells for culturing. The ATF5 recombinant monoclonal antibody was purified from the supernatant of the cell culture using affinity chromatography and rigorously verified. It can be utilized in ELISA, WB, and IHC experiments for the detection of human and rat ATF5 proteins.
ATF5 is a transcription factor protein that plays a role in the regulation of gene expression. It belongs to the ATF/CREB family of transcription factors and is involved in various cellular processes such as apoptosis, cell differentiation, and proliferation. ATF5 has been implicated in the regulation of neuronal survival and differentiation, as well as in the pathogenesis of various diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. It has also been shown to be involved in the response to cellular stress such as oxidative stress, hypoxia, and endoplasmic reticulum stress.
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