BAG3 Antibody

Code CSB-RA002531A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate
    All lanes: BAG3 antibody at 0.73μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 62 KDa
    Observed band size: 80 KDa

  • IHC image of CSB-RA002531A0HU diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Uniprot No. O95817
Target Names BAG3
Alternative Names BAG 3 antibody; BAG family molecular chaperone regulator 3 antibody; BAG-3 antibody; Bag3 antibody; BAG3_HUMAN antibody; Bcl 2 binding protein antibody; Bcl-2-associated athanogene 3 antibody; Bcl-2-binding protein Bis antibody; BCL2 associated athanogene 3 antibody; BCL2 binding athanogene 3 antibody; BIS antibody; CAIR 1 antibody; Docking protein CAIR 1 antibody; Docking protein CAIR-1 antibody; MFM6 antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human BAG3
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70
Gene References into Functions
  1. There was no association of SNPs in ADRB1, GRK5 and BAG3 genes with Takotsubo cardiomyopathy. PMID: 29514624
  2. These findings provide a molecular basis for understanding of BAG3dependent cell proliferation and survival from the aspect of alteration of gene expression. PMID: 30106105
  3. BAG3 can modulate the levels, localization or activity of its partner proteins, thereby regulating major cell pathways and functions, including apoptosis, autophagy, mechanotransduction, cytoskeleton organisation, motility. PMID: 28864347
  4. Genetic and pharmacological interference with BAG3 is capable to resensitize TNBC cells to treatment. PMID: 29462756
  5. The HSF1-BAG3-Mcl-1 signal axis is critical for protection of mutant KRAS colon cancer cells from AUY922-induced apoptosis. PMID: 29068469
  6. The Hsp70-Bag3 complex therefore functions as an important signaling node that senses proteotoxicity and triggers multiple pathways that control cell physiology, including activation of protein aggregation. PMID: 29987014
  7. study revealed oncogenic roles of BAG3 in chondrosarcoma and provided mechanisms that the BAG3-modulated the expression of RUNX2 through upregulation of beta-catenin. PMID: 29484408
  8. BAG3 interacted with CXCR4 mRNA and promoted its expression via its coding and 3'-untranslational regions. PMID: 28703799
  9. Silencing of HSPB8 markedly decreased the mitotic levels of BAG3 in HeLa cells, supporting its crucial role in BAG3 mitotic functions. The results support a role for the HSPB8-BAG3 chaperone complex in quality control of actin-based structure dynamics that are put under high tension, notably during cell cytokinesis. PMID: 28275944
  10. Our findings suggest that high levels of BIS expression might confer stem-cell-like properties on cancer cells through STAT3 stabilization PMID: 27145367
  11. modulation of proteostasis is a distinct biological function of sAPPalpha and does not require surface-bound holo-APP. PMID: 26526841
  12. higher levels of BAG3 were observed in hypertensive patients compared to healthy controls, and even higher levels in hypertensive diabetic patients compared to healthy subjects. PMID: 28696030
  13. BAG3 directly stabilizes hexokinase 2 mRNA and promotes aerobic glycolysis in pancreatic cancer cells. PMID: 29114069
  14. These results indicated that at least some oncogenic functions of BAG3 were mediated through posttranscriptional regulation of Skp2 via antagonizing suppressive action of miR-21-5p in ovarian cancer cells. PMID: 28624440
  15. Familial suffering of Dilated cardiomyopathy and carrying a heterozygous large deletion in the BAG3 gene.This gene encodes BCL2-associated athanogene 3 protein. PMID: 27736720
  16. BAG3 mutations are associated with DCM phenotypes. BAG3 should be added to cardiomyopathy gene panels for screening of DCM patients, and patients previously considered gene elusive should undergo sequencing of the BAG3 gene. PMID: 28211974
  17. The spatial regulation of mTORC1 exerted by BAG3 apparently provides the basis for a simultaneous induction of autophagy and protein synthesis to maintain the proteome under mechanical strain. PMID: 27756573
  18. This study therefore identifies both BAG3 reduction and autophagy promotion as potential therapies for FLNC(W2710X) myofibrillar myopathy, and identifies protein insufficiency due to sequestration, compounded by impaired autophagy, as the cause. PMID: 26969713
  19. variants in TNNT2 and BAG3 are associated with a high propensity to life-threatening cardiomyopathy presenting from childhood and young adulthood. PMID: 28669108
  20. It has been demonstrated that HSPB8-BAG3-HSP70 ensures the functionality of stress granules and restores proteostasis by targeting defective ribosomal products for degradation. PMID: 27570075
  21. Study report p.H243Tfr*64_BAG3 as a novel pathogenic variation responsible for familial dilated cardiomyopathy. This variation correlates with a more severe phenotype of the disease, mainly in younger individuals. PMID: 27391596
  22. The authors propose that the chaperone-mediated autophagy function of BAG3 represents a specific host defense strategy to counteract the function of VP40 in promoting efficient egress and spread of virus particles. PMID: 28076420
  23. BAG3 bound to Hsp70 at the same time as Hsp22, Hsp27, or alphaB-crystallin, suggesting that it might physically bring the chaperone families together into a complex. PMID: 27884606
  24. These results suggest that Bag1 and Bag3 control the stability of the Hsc70-client complex using at least two distinct protein-protein contacts, providing a previously under-appreciated layer of molecular regulation in the human Hsc70 system. PMID: 27474739
  25. we report the first mammalian model of a single amino acid mutation of BAG3 (P209L) in exon 3 of Bag3 associated with the development of muscle disease with left ventricular dysfunction and heart failure PMID: 27321750
  26. High BAG3 expression Correlates with Sebaceous Gland Carcinoma of the Eyelid. PMID: 28373462
  27. These results indicate a possible role for BAG3 protein in the maintenance of cell survival in endometrioid endometrial cancer. PMID: 27414463
  28. By showing transcription factor HSF1 activation, we demonstrated that HCA induces the expression of BAG3 through HSF1 activation. More importantly, knockdown of BAG3 expression using siRNA largely inhibited HCA-induced apoptosis, suggesting that BAG3 is actively involved in HCA-induced cancer cell death PMID: 27922674
  29. These results provide further insight into the molecular mechanisms involved in the enhancement of hyperthermia sensitivity by the silencing of BAG3 in human oral squamous cell carcinoma cells. PMID: 27245201
  30. high expression of BAG3 was detected in a majority of medulloblastoma tissues and predicted poor outcome for medulloblastoma patients. PMID: 27456361
  31. in the present study, we demonstrated that BAG3 overexpression plays a critical role in cell proliferation, migration, and invasion of colorectal cancer. Our data suggests targeted inhibition of BAG3 may be useful for patients with colorectal cancer PMID: 26577854
  32. BAG3 maintains the basal amount of LC3B protein by controlling the translation of its mRNA in HeLa and HEK293 cells. PMID: 26654586
  33. Data indicate a tumor suppressor-like function of Bcl-2 associated athanogene 3 (BAG3) via direct interaction with glucose 6 phosphate dehydrogenase (G6PD) in hepatocellular carcinomas (HCCs) at the cellular level. PMID: 26621836
  34. BAG3 is associated with Z-disc maintenance. PMID: 26545904
  35. BAG3 is a suitable target for combined therapies aimed at synergistically inducing apoptosis in bladder cancer. PMID: 26100943
  36. Bis expression was higher in squamous cell carcinoma than in adenocarcinoma in Lung Cancer. PMID: 25968616
  37. BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages. PMID: 26522614
  38. BAG3-mediated miRNA let-7g and let-7i inhibit proliferation and enhance apoptosis of human esophageal carcinoma cells by targeting the drug transporter ABCC10 and modulates cisplatin resistance. PMID: 26655271
  39. This newly described ERa-mediated and estrogen response element (ERE)-independent non-canonical autophagy pathway, which involves the function of BAG3 and provides stress resistance in our model systems. PMID: 26158518
  40. BAG3 protein loci is involved in the pathophysiology of systolic heart failure. PMID: 25889438
  41. MiR-143 enhanced the tumor suppressive effect of shikonin partly through the regulation of BAG3 in glioblastoma stem cells. PMID: 26541455
  42. The Hsp70-Bag3 interaction may be a promising, new target for anticancer therapy. PMID: 25564440
  43. BAG3 mutations should be considered even in cases with a mild phenotype or an adult onset PMID: 25208129
  44. The stress response protein BAG3 may be prognostic for death in patients with acutely decompensated heart failure. PMID: 25753466
  45. BAG-3 expression correlated with increased HSP70 expression in a subset of systemic T cell lymphoma cases co-expressing the CD30 antigen. PMID: 24492285
  46. To examine the relationships among BAG3, miR-29b and MMP2 in endometrioid adenocarcinoma cells. PMID: 25738313
  47. Upon glucose stimulation BAG3 is phosphorylated by FAK and dissociates from SNAP-25 allowing the formation of the SNARE complex, destabilization of the F-actin network and insulin release. PMID: 25766323
  48. Studies indicate that heat shock protein Hsp70 (Hsp70) regulates multiple pathways in cancer cells via interaction with Bcl2-associated athanogene 3 protein (BAG3) co-chaperone. PMID: 25347739
  49. BAG3 plays a key role in the processing of the nucleocytoplasmic shuttling of HSF1 upon heat stress PMID: 26159920
  50. HIV-1 Tat protein is able to stimulate autophagy through increasing BAG3 levels in glial cells. PMID: 25483098
  51. Bag3-MVP is an important complex that regulates a potent prosurvival signaling pathway and contributes to chemotherapy resistance in breast cancer PMID: 24997994
  52. Knockdown of BAG3 also significantly suppressed motion and invasion of HK2 cells mediated by FGF-2 PMID: 25361773
  53. findings showed that the P209L mutation causes BAG3 to aggregate; proposed that the gradual loss of available BAG3(wt) and BAG3(P209L) proteins results in insufficiency leading to myofibrillar disintegration PMID: 25273835
  54. BAG3 binding to the ubiquitinated clients occurs through the BAG domain, in competition with BAG1 PMID: 25046115
  55. A subset of SCLCs over express BAG3 that exerts an anti-apoptotic effect resulting in resistance to chemotherapy. PMID: 25149536
  56. These results identify a subgroup of stage III melanoma patients, that is, patients with 2-3 positive lymph nodes, whose clinical behavior is influenced by the expression of the anti-apoptotic BAG3 protein. PMID: 24722298
  57. Disrupts the Hsp70-Bag3 interaction. PMID: 24994713
  58. Findings reveal a novel role for BAG3 as host protein contributing to HPV18 E6-activated pro-survival strategies. PMID: 25175321
  59. BAG3 carries highly penetrant DCM mutations that are associated with a worse prognosis characterized by earlier age of onset. PMID: 25448463
  60. The results suggested that the expression level of BAG3 and HIF-1 alpha is efficient prognostic parameters in patients with HCC after liver transplantation. PMID: 24895585
  61. BAG3 point mutations and large deletions are relatively frequent cause of dilated cardiomyopathy PMID: 25008357
  62. These results indicate BAG3 as a regulator of ZEB1 expression in epithelial-mesenchymal transition and as a regulator of metastasis in thyroid cancer cells. PMID: 24577090
  63. Diminished levels of BAG3 protein may be associated with both familial and non-familial forms of dilated cardiomyopathy. PMID: 24623017
  64. Bis knockdown resulted in a significant decrease in the migration and invasion of A172 glioma cells. Furthermore, Bis knockdown notably decreased TPA-induced matrix metalloproteinase-9 (MMP-9) activity and mRNA expression. PMID: 24286317
  65. Missense mutations in antiapoptotic bag3 protein are found in patients with tako-tsubo cardiomyopathy. PMID: 23582692
  66. Functional studies demonstrated that BAG3 interacts with the proteasome and modulates its activity, sustaining cell survival and underlying resistance to therapy through the down-modulation of apoptosis. PMID: 23824909
  67. BAG3 was overexpressed in hepatocellular carcinoma. BAG3 knockdown resulted in reduction in migration and invasion of cells, which was linked to reversion of epithelial-mesenchymal transition. PMID: 24365746
  68. Data suggest that BAG3 expression is increased in chronic lymphocytic leukemia (CLL) cells compared with normal B-cells; knocking down BAG3 expression results in increased apoptosis and decreased cell migration in primary CLL cells. PMID: 23978946
  69. we measured the binding of human Hsp72 (HSPA1A) to BAG1, BAG2, BAG3, and the unrelated NEF Hsp105. These studies revealed a clear hierarchy of affinities: BAG3 > BAG1 > Hsp105 >> BAG2. PMID: 24318877
  70. aberrant Bag-3 expression might be involved in colorectal adenoma-adenocarcinoma sequence and subsequent progression PMID: 23553442
  71. these results suggested that through direct interaction BAG3 could prevent the antiapoptotic effect of GRP78 upon genotoxic stress. PMID: 24080088
  72. BAG3 stabilized JunD mRNA. PMID: 24140207
  73. Noncanonical autophagy mediated by BAG3 suppresses responsiveness of HepG2 cells to proteasome inhibitors PMID: 23575457
  74. BAG3 is a novel substrate of PKCdelta, and PKCdelta-mediated phosphorylation of BAG3 is implicated in EMT and invasiveness of thyroid cancer cells. PMID: 23108398
  75. we identified Caspase 3, BAG1 and BAG3 as key targets of ERalpha in neuronal cells that may play a role in ERalpha-mediated neuroprotection. PMID: 23271418
  76. Autophagosome formation during chaperone-assisted selective autophagy depends on an interaction of BAG3 with synaptopodin-2. PMID: 23434281
  77. Hyperthermia sensitivity in human oral squamous cell carcinoma cells is enhanced upon silencing of BAG3 and inhibition of the JNK pathway. PMID: 23395971
  78. the first Chinese case of Bag3opathy so far reported. PMID: 21361913
  79. BAG3 is expressed in endothelial cells. PMID: 22310281
  80. Studies indicate BAG3-mediated Mcl-1 stabilization as a potential target for cancer drug discovery. PMID: 23341456
  81. BAG3 expression level might be a potential marker for prediction of patient outcome. PMID: 22944597
  82. Data suggest that by investigating the interplay between large T-antigen (T-Ag) and Bag3 may provid better understanding on the development of JCV-associated diseases. PMID: 22984599
  83. BAG3 balances autophagic degradation and YAP/TAZ-mediated transcription of filamin in mechanically strained cells and tissues. BAG3 uses its WW domain to associate with SYNPO2 during autophagosome formation or to bind the YAP/TAZ-inhibitors LATS1/2 and AMOTL1/2 during transcription regulation. This dual function is essential for tissue maintenance and for cell adhesion, migration and proliferation in mammals. PMID: 23434281
  84. This report confirms the association of giant axonal neuropathy with BAG3-associated myofibrillar myopathy PMID: 22734908
  85. findings show that during heat shock recovery NF-kappaB activates selective removal of misfolded or aggregated proteins by controlling expression of BAG3 and HSPB8 and by modulating the level of the BAG3-HspB8 complex PMID: 22302993
  86. we observed upregulation of HSPB8 and BAG3 selectively in astrocytes located within the degenerated areas of patients with protein aggregation diseases PMID: 21696420
  87. We report here two heterozygous missense mutations of BAG3 found in familial DCM, which cause abnormal Z-disc assembly and increase the sensitivity to apoptosis in the cardiomyocytes. PMID: 21898660
  88. BAG3 protein sustains anaplastic thyroid tumor growth in vitro and in vivo. The underlying molecular mechanism appears to rely on BAG3 binding to BRAF, thus protecting it from proteasome-dependent degradation. PMID: 22072743
  89. non-small cell lung cancer cells were protected from apoptosis through increasing Bag3 expression and consequently promoted the expression of Bcl-XL and Bcl-2. PMID: 21971866
  90. BAG3 is expressed in a proportion of melanoma and in the majority of metastases, so it may be involed in tumor development. PMID: 21850027
  91. the data support that BAG3 is induced by LPS via JNK and NF-kappaB-dependent signals, and involved in monocytic cell-extracellular matrix interaction, suggesting that BAG3 may have a role in the host response to LPS stimulation PMID: 22020323
  92. The results show that rare mutations in BAG3 contribute to monogenic forms of the disease, while common variant(s) in the same gene are implicated in sporadic DCM PMID: 21459883
  93. BAG3 was identified as a marker of electromagnetic field-induced stress. These results suggest that BAG3 induction by an electromagnetic field may contribute to melanoma cell survival and/or resistance to therapy. PMID: 21302292
  94. BAG3, although negative in normal brain tissues, was highly expressed in astrocytic tumors and increasingly expressed in more aggressive types of cancer; it was particularly high in glioblastomas. PMID: 21561597
  95. bag3 expression in chronic lymphocytic leukemia patients is markedly higher than that in normal controls, while high bag3 level in CML patients is probably related with drug resistance, but is not related with clinically established prognostic factors. PMID: 20723284
  96. The complexes formed by Bag3 and HspB8 might have variable stoichiometry and can participate in different processes including clearing of the cell from improperly folded proteins. PMID: 21767525
  97. Multifaceted interactions underlie BAG3 ability to modulate apoptosis, development, cytoskeleton organization and autophagy. [Review] PMID: 21472004
  98. BAG3 promotes the emergence of castration resistant prostate cancer by modulating IKKalpha nuclear translocation. PMID: 21451574
  99. These findings indicate a novel function for BAG3 in inhibiting protein aggregation caused by the genetic mutation of CRYAB responsible for human myofibrillar myopathy. PMID: 21423662
  100. aggresome-targeting by BAG3 is distinct from previously described mechanisms, as it does not depend on substrate ubiquitination PMID: 21252941

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Involvement in disease Myopathy, myofibrillar, 6 (MFM6); Cardiomyopathy, dilated 1HH (CMD1HH)
Subcellular Location Nucleus, Cytoplasm
Database Links

HGNC: 939

OMIM: 603883

KEGG: hsa:9531

STRING: 9606.ENSP00000358081

UniGene: Hs.523309

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