Code | CSB-RA291376A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
CASP2 recombinant monoclonal antibody is produced using a combination of protein and DNA recombinant technologies. The first step involves immunizing mice with a synthesized peptide from human CASP2, followed by the removal of the spleen cells and extraction of total RNA under aseptic conditions. The cDNA obtained by reverse transcription of RNA is then used as a template for PCR amplification of the CASP2 antibody gene. The amplified gene is inserted into a vector and transfected into host cells for culture. The CASP2 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography and rigorously verified for its specificity and sensitivity in ELISA and WB experiments for the detection of human CASP2 protein.
The CASP2 protein is a protease that plays an important role in programmed cell death, or apoptosis. CASP2 is unique among the caspase family in that it contains a long prodomain region that can inhibit its activity until specific signaling events occur. When cells are subjected to certain types of stress, such as DNA damage, the prodomain of CASP2 is cleaved, allowing the active protease to cleave and activate downstream targets that promote cell death. CASP2 has been implicated in other cellular processes, such as DNA repair and cell cycle regulation.
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