Product Details

Uniprot No.

Target Names

Alternative Names

NAD-dependent protein deacylase sirtuin-5, mitochondrial (EC 3.5.1.-) (Regulatory protein SIR2 homolog 5) (SIR2-like protein 5), SIRT5, SIR2L5

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human SIRT5

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SIRT5 belongs to the sirtuin family of NAD-dependent deacylases and plays a distinctive role in mitochondrial metabolism. Unlike other sirtuins that primarily remove acetyl groups, SIRT5 demonstrates robust desuccinylase and demalonylase activity, making it a critical regulator of metabolic pathways including fatty acid oxidation, the urea cycle, and ketone body production. Its mitochondrial localization positions SIRT5 as a key player in cellular energy homeostasis, with growing evidence linking its dysregulation to metabolic disorders, cardiovascular disease, and cancer progression.

This recombinant monoclonal antibody, generated from clone 9H12, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and produced through recombinant expression in rabbit host cells, you can expect consistent performance across lots, eliminating the variability that often complicates experiments using traditional hybridoma-derived antibodies. The antibody was raised against a synthetic peptide derived from human SIRT5, ensuring precise epitope targeting.

Validation in immunohistochemistry demonstrates reliable detection in paraffin-embedded human kidney tissue, where SIRT5 expression is particularly relevant given the kidney's high metabolic demands. Using a Leica Bond system with citrate buffer antigen retrieval and HRP-polymer detection, clear staining was achieved at 1:100 dilution, with a recommended working range of 1:50 to 1:200. The antibody is also validated for ELISA applications, providing flexibility for both tissue-based localization studies and quantitative protein analysis.

Whether investigating mitochondrial dysfunction in metabolic disease, exploring epigenetic regulation in cancer models, or examining cardiovascular pathophysiology, this SIRT5 antibody delivers the specificity and consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

Gene References into Functions

In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5iso1, SIRT5iso2, SIRT5iso3, and SIRT5iso4. SIRT5(iso1-3) were mitochondria-localized, while SIRT5(iso4) localized mainly in cytoplasm. SIRT5(iso2-4) had little deacylase activity comparing with SIRT5(iso1). PMID: 29932920
miR-299-3p suppresses migration, invasion and proliferation of Hepatocellular carcinoma cells via directly targeting SIRT5 PMID: 30170358
High SIRT5 expression is associated with Cancer Cell Proliferation. PMID: 29180469
The DNA variants and SNPs identified in acute Myocardial infection (AMI) patients may change SIRT5 level by affecting activity of SIRT5 gene promoter, contributing to the AMI development as a risk factor. PMID: 29981421
In this study, we summarize biological functions of SIRT5 reported in normal tissues and in cancer and discuss potential mechanisms whereby SIRT5 may impact tumorigenesis, particularly focusing on its reported roles in metabolic reprogramming. Finally, we review current efforts to target SIRT5 pharmacologically PMID: 28707979
The roles of the CDK2/SIRT5 axis in gastric cancer. PMID: 29896817
SIRT5 gene has an important regulatory role in liver carcinogenesis, and may function as a novel potential therapeutic target for HCC. PMID: 29115436
This study showed that SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1. PMID: 29416026
Results show that SIRT5 binds to, desuccinylates and inhibits PKM2 activity. Increased levels of reactive oxygen species (ROS) decreases succinylation and activity of PKM2 by increasing its binding to SIRT5. Moreover, inhibition of SIRT5 suppresses tumor cell proliferation through desuccinylation of PKM2 K498. PMID: 28036303
The function of the three mitochondrial sirtuins (SIRT3, SIRT4, SIRT5) and their role in disease are reviewed. PMID: 27164052
Our study uncovers a SIRT5-dependent mechanism that regulates cellular NADPH homeostasis and redox potential by promoting IDH2 desuccinylation and G6PD deglutarylation. PMID: 27113762
Results demonstrated presence of endogenous SIRT5 in mitochondria of cultured SH-EP cells, identified down-regulation of cellular oxidative stress by SIRT5 as one of the possible mechanisms mediating the anti-apoptotic effect of SIRT5 in SH-EP cells. PMID: 27577743
Data (including data from studies using knockout mice) suggest that SIRT5 is targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin to promote respiratory chain function, particularly Complex I and Complex II; SIRT5 expression is observed in inner mitochondrial membrane of periportal hepatocytes. PMID: 28458255
a side chain-to-side chain cyclic pentapeptide harboring a central N(epsilon)-carboxyethyl-thiocarbamoyl-lysine residue behaved as a strong and selective (versus human SIRT1/2/3/6) inhibitor against human SIRT5-catalyzed deacylation reaction. PMID: 27626398
mutual cooperation between Y102 and R105 residues in promoting the desuccinylation versus deacetylation reaction in SIRT5. PMID: 27023330
Data showed that SIRT5 was involved in protein post-translational modifications through its potent demalonylase, desuccinylase, and deglutarylase activities. Also, the protein was found in the mitochondrial, cytoplasmic, and nuclear compartments. [review] PMID: 25717114
Results show that mitochondrial sirtuins SIRT3, SIRT4, and SIRT5 can promote increased mitochondrial respiration and cellular metabolism and respond to excess glucose by inducing a coordinated increase of glycolysis and respiration. PMID: 25165814
This study demonstrated that SIRT15 downregulation in the liver of non-alcoholic fatty liver disease patients. PMID: 25361925
Results suggest a role for SIRT5 in influencing oocyte quality and in vitro fertilization outcomes. PMID: 23978077
SIRT1/SIRT5-mediated PML deacetylation plays a role in the regulation of cancer cell survival. PMID: 25032863
The results reveal the Sirt5 substrate acyl selectivity and its molecular basis and enable the design of inhibitors for Sirt5. PMID: 25111069
High SIRT5 expression facilitates cancer cell growth and drug resistance in non-small cell lung cancer. PMID: 25070488
Expression of SIRT5 in the entorhinal cortex, hippocampus, and white matter increases during the progression of Alzheimer disease. It is seen in activated microglia. PMID: 24464653
These results reveal a novel post-translational regulation of SOD1 by means of succinylation and SIRT5-dependent desuccinylation, which is important for the growth of lung tumor cells. PMID: 24140062
Downregulation of SIRT5 is associated with head and neck squamous cell carcinoma. PMID: 23475622
Analysis of Sirt5 structures and activity data suggest that an Arg/succinate interaction is the molecular basis of the differential nicotinamide sensitivities of the two Sirt5 activities. PMID: 23028781
The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). PMID: 22767592
NAD(+)-dependent SIRT deacetylase has a role in regulating the expression of mitochondrial steroidogenic P450 PMID: 22585829
we demonstrate that Sirt5, a member of the class III lysine deacetylases, can catalyze lysine demalonylation and lysine desuccinylation reactions both in vitro and in vivo PMID: 21908771
study found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro; protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo PMID: 22076378
SIRT5 represents a risk factor for mitochondrial dysfunction-related diseases, including Parkinson's, through accelerated molecular aging of disease-related genes. PMID: 20887790
these results indicate that human SIRT5 potentially controls various primate-specific functions via two isoforms with different intracellular localizations or stabilities. PMID: 21143562
SIRT5 consists of eight exons and is found in two isoforms, which encode a 310 aa and a 299 aa protein, respectively. PMID: 16484774
Analyses did not yield convincing evidence for associations of schizophrenia with SIRT5. PMID: 16827919
Results describe the substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. PMID: 18680753

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Subcellular Location

Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.; [Isoform 1]: Cytoplasm. Mitochondrion.; [Isoform 2]: Mitochondrion.

Protein Families

Sirtuin family, Class III subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 14933

OMIM: 604483

KEGG: hsa:23408

STRING: 9606.ENSP00000368552

UniGene: Hs.567431

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Code	CSB-RA156557A0HU
Size	US$210
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Image	IHC image of CSB-RA156557A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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SIRT5 Recombinant Monoclonal Antibody

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Target Background