SIRT5 Antibody

Code CSB-RA156557A0HU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • IHC image of CSB-RA156557A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details

Uniprot No. Q9NXA8
Target Names SIRT5
Alternative Names NAD-dependent protein deacylase sirtuin-5, mitochondrial (EC 3.5.1.-) (Regulatory protein SIR2 homolog 5) (SIR2-like protein 5), SIRT5, SIR2L5
Species Reactivity Human
Immunogen A synthesized peptide derived from human SIRT5
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 9H12
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins
Gene References into Functions
  1. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5iso1, SIRT5iso2, SIRT5iso3, and SIRT5iso4. SIRT5(iso1-3) were mitochondria-localized, while SIRT5(iso4) localized mainly in cytoplasm. SIRT5(iso2-4) had little deacylase activity comparing with SIRT5(iso1). PMID: 29932920
  2. miR-299-3p suppresses migration, invasion and proliferation of Hepatocellular carcinoma cells via directly targeting SIRT5 PMID: 30170358
  3. High SIRT5 expression is associated with Cancer Cell Proliferation. PMID: 29180469
  4. The DNA variants and SNPs identified in acute Myocardial infection (AMI) patients may change SIRT5 level by affecting activity of SIRT5 gene promoter, contributing to the AMI development as a risk factor. PMID: 29981421
  5. In this study, we summarize biological functions of SIRT5 reported in normal tissues and in cancer and discuss potential mechanisms whereby SIRT5 may impact tumorigenesis, particularly focusing on its reported roles in metabolic reprogramming. Finally, we review current efforts to target SIRT5 pharmacologically PMID: 28707979
  6. The roles of the CDK2/SIRT5 axis in gastric cancer. PMID: 29896817
  7. SIRT5 gene has an important regulatory role in liver carcinogenesis, and may function as a novel potential therapeutic target for HCC. PMID: 29115436
  8. This study showed that SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1. PMID: 29416026
  9. Results show that SIRT5 binds to, desuccinylates and inhibits PKM2 activity. Increased levels of reactive oxygen species (ROS) decreases succinylation and activity of PKM2 by increasing its binding to SIRT5. Moreover, inhibition of SIRT5 suppresses tumor cell proliferation through desuccinylation of PKM2 K498. PMID: 28036303
  10. The function of the three mitochondrial sirtuins (SIRT3, SIRT4, SIRT5) and their role in disease are reviewed. PMID: 27164052
  11. Our study uncovers a SIRT5-dependent mechanism that regulates cellular NADPH homeostasis and redox potential by promoting IDH2 desuccinylation and G6PD deglutarylation. PMID: 27113762
  12. Results demonstrated presence of endogenous SIRT5 in mitochondria of cultured SH-EP cells, identified down-regulation of cellular oxidative stress by SIRT5 as one of the possible mechanisms mediating the anti-apoptotic effect of SIRT5 in SH-EP cells. PMID: 27577743
  13. Data (including data from studies using knockout mice) suggest that SIRT5 is targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin to promote respiratory chain function, particularly Complex I and Complex II; SIRT5 expression is observed in inner mitochondrial membrane of periportal hepatocytes. PMID: 28458255
  14. a side chain-to-side chain cyclic pentapeptide harboring a central N(epsilon)-carboxyethyl-thiocarbamoyl-lysine residue behaved as a strong and selective (versus human SIRT1/2/3/6) inhibitor against human SIRT5-catalyzed deacylation reaction. PMID: 27626398
  15. mutual cooperation between Y102 and R105 residues in promoting the desuccinylation versus deacetylation reaction in SIRT5. PMID: 27023330
  16. Data showed that SIRT5 was involved in protein post-translational modifications through its potent demalonylase, desuccinylase, and deglutarylase activities. Also, the protein was found in the mitochondrial, cytoplasmic, and nuclear compartments. [review] PMID: 25717114
  17. Results show that mitochondrial sirtuins SIRT3, SIRT4, and SIRT5 can promote increased mitochondrial respiration and cellular metabolism and respond to excess glucose by inducing a coordinated increase of glycolysis and respiration. PMID: 25165814
  18. This study demonstrated that SIRT15 downregulation in the liver of non-alcoholic fatty liver disease patients. PMID: 25361925
  19. Results suggest a role for SIRT5 in influencing oocyte quality and in vitro fertilization outcomes. PMID: 23978077
  20. SIRT1/SIRT5-mediated PML deacetylation plays a role in the regulation of cancer cell survival. PMID: 25032863
  21. The results reveal the Sirt5 substrate acyl selectivity and its molecular basis and enable the design of inhibitors for Sirt5. PMID: 25111069
  22. High SIRT5 expression facilitates cancer cell growth and drug resistance in non-small cell lung cancer. PMID: 25070488
  23. Expression of SIRT5 in the entorhinal cortex, hippocampus, and white matter increases during the progression of Alzheimer disease. It is seen in activated microglia. PMID: 24464653
  24. These results reveal a novel post-translational regulation of SOD1 by means of succinylation and SIRT5-dependent desuccinylation, which is important for the growth of lung tumor cells. PMID: 24140062
  25. Downregulation of SIRT5 is associated with head and neck squamous cell carcinoma. PMID: 23475622
  26. Analysis of Sirt5 structures and activity data suggest that an Arg/succinate interaction is the molecular basis of the differential nicotinamide sensitivities of the two Sirt5 activities. PMID: 23028781
  27. The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). PMID: 22767592
  28. NAD(+)-dependent SIRT deacetylase has a role in regulating the expression of mitochondrial steroidogenic P450 PMID: 22585829
  29. we demonstrate that Sirt5, a member of the class III lysine deacetylases, can catalyze lysine demalonylation and lysine desuccinylation reactions both in vitro and in vivo PMID: 21908771
  30. study found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro; protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo PMID: 22076378
  31. SIRT5 represents a risk factor for mitochondrial dysfunction-related diseases, including Parkinson's, through accelerated molecular aging of disease-related genes. PMID: 20887790
  32. these results indicate that human SIRT5 potentially controls various primate-specific functions via two isoforms with different intracellular localizations or stabilities. PMID: 21143562
  33. SIRT5 consists of eight exons and is found in two isoforms, which encode a 310 aa and a 299 aa protein, respectively. PMID: 16484774
  34. Analyses did not yield convincing evidence for associations of schizophrenia with SIRT5. PMID: 16827919
  35. Results describe the substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. PMID: 18680753

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Subcellular Location Mitochondrion matrix, Mitochondrion intermembrane space, Cytoplasm, cytosol, Nucleus
Protein Families Sirtuin family, Class III subfamily
Tissue Specificity Widely expressed.
Database Links

HGNC: 14933

OMIM: 604483

KEGG: hsa:23408

STRING: 9606.ENSP00000368552

UniGene: Hs.567431

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