Trx-Tag Monoclonal Antibody

Derived from the enzyme thioredoxin, the Trx tag is a highly effective fusion partner renowned for its exceptional ability to enhance the solubility of recombinant proteins, particularly in bacterial expression systems. Beyond its role in promoting proper protein folding, the Trx tag confers valuable thermostability, allowing constructs to withstand high-temperature treatments often used to precipitate contaminants during affinity purification. This dual functionality makes it an invaluable tool for expressing challenging proteins, including membrane proteins and inclusion bodies.

The Mouse Anti-Trx-Tag Monoclonal Antibody (CSB-MA000173, IgG isotype) provides precise and monospecific detection for these fusion constructs. Produced via robust hybridoma technology and generated against a recombinant protein immunogen, this antibody ensures outstanding specificity and consistent lot-to-lot performance.

Validated for both ELISA and Western Blot (WB) applications, the reagent exhibits high sensitivity and a strong binding affinity. As demonstrated in the Western Blot analysis, the antibody successfully detects 1 µg of the Trx fusion protein at high working dilutions of 1:5,000 and 1:10,000. The intense, well-defined bands observed at both concentrations, with no visible background interference or protein degradation, confirm the antibody's excellent immunoreactivity and superior signal-to-noise ratio. Its high titer translates into significant cost savings for high-throughput laboratory applications.

This monoclonal antibody is an essential asset for researchers focused on protein expression and purification. It serves as a critical tool for verifying successful Trx fusion protein expression, accurately assessing molecular weight, and monitoring the efficiency of downstream biochemical assays. With its proven reliability, the Trx-Tag Monoclonal Antibody supports robust scientific discovery in molecular biology and structural proteomics.