Recombinant Human H (+)/Cl (-) exchange transporter 3 (CLCN3)

Code CSB-CF005482HU(A4)
Size $2160
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
CLCN3; H(+/Cl(- exchange transporter 3; Chloride channel protein 3; ClC-3; Chloride transporter ClC-3
Homo sapiens (Human)
in vitro E.coli expression system
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
93.2 kDa
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
18-23 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons. The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.; Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons.
Gene References into Functions
  1. Unique oligomerization properties of ClC-4 permit regulated targeting of ClC-4 to various endosomal compartment systems via expression of different ClC-3 splice variants. PMID: 28972156
  2. we demonstrated that ClC-3 can arrest the cell cycle at the G1 phase to inhibit cell proliferation, suggesting that ClC-3 has the potential to be a novel target for hepatocellular carcinoma (HCC) therapy and potentially improve the prognosis of HCC patients PMID: 29749557
  3. CLC-3 may get involved in proliferation, invasion, and migration of ovarian cancer cells and thus may be a useful therapeutic target. PMID: 29787012
  4. ClC-3 is a potential target of 17beta-estradiol and is modulated by the ERalpha in breast cancer cells. PMID: 28419445
  5. Study provided novel and compelling evidence for the functional role of the unique CLC-3, which are significantly upregulated during ischemia, in the protection of the heart under stress PMID: 27064645
  6. ClC-3 promotes endometriotic cell migration and invasion. PMID: 26965430
  7. these results demonstrated that ClC-3 is involved in the proliferation and migration of osteosarcoma cell PMID: 25973047
  8. Data indicate that cytoplasmic chloride channel-3 (ClC-3) plays an active and key role in tumor metastasis and may be a valuable prognostic biomarker and a therapeutic target to prevent tumor spread. PMID: 25537517
  9. CLC3 is required in the activation and migration of human blood eosinophils. PMID: 25514499
  10. Authors summarize the function of CLC-3 in cancer and discuss the mechanisms by which CLC-3 contributes to proliferation, apoptosis and drug resistance in cancer cells. [Review] PMID: 25421907
  11. swelling-activated Cl currents and CLC-3 play a role in pulmonary artery smooth muscle cell proliferation, but CLC-3 channels do not underlie swelling in these cells PMID: 24284495
  12. ClC-3 deficiency inhibited Ang II-induced EPC apoptosis via suppressing ROS generation derived from NADPH oxidase. PMID: 23873092
  13. Suggest that ClC-3 suppression causes the inhibition of Akt and autophagy, which can enhance the therapeutic benefit of cisplatin in U251 cells. PMID: 23408563
  14. K(Ca)3.1 and ClC-3 are expressed in tissue samples obtained from patients diagnosed with grade IV gliomas. Both K(Ca)3.1 and ClC-3 colocalize to the invading processes of glioma cells PMID: 23345219
  15. Data indicate in umbilical vein endothelial cells transfected with ClC-3 siRNA showed activation of NF-kappaB. PMID: 23006728
  16. results reveal a cell cycle-dependent change of the subcellular distribution of ClC-3 and strongly suggest that ClC-3 has nucleocytoplasmic shuttling dynamics that may play key regulatory roles during different stages of the cell cycle in tumor cells. PMID: 22371056
  17. ClC-3 is a candidate of the channel proteins that mediate or regulate the acid-activated chloride current in nasopharyngeal carcinoma cells. PMID: 22496242
  18. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma. PMID: 22108225
  19. ClC-3 may be a main component of background chloride channels which can be activated under isotonic conditions by autocrine/paracrine ATP through purinergic receptor pathways; the background current is involved in maintenance of basal cell volume. PMID: 21792908
  20. ClC-3 is involved in the activation of volume-activated chloride currents but not of stretch-activated chloride currents in hepatocellular carcinoma cells. PMID: 20945353
  21. High ClC-3 is associated with extensive migration and invasion in glioma. PMID: 21115901
  22. ClC-3 is an important molecular component underlying VSOACs and the RVD process in HeLa cells PMID: 12183454
  23. results show that the ClC-3B PDZ-binding isoform resides in the Golgi where it co-localizes with a small amount of CFTR (cystic fibrosis transmembrane conductance regulator) PMID: 12471024
  24. results suggest a fundamental role of endogenous ClC-3 in the swelling-activated Cl- channels function and cell volume regulation in human gastric epithelial cells PMID: 12842831
  25. ClC-3 and ZnT3 reside in a common vesicle population where they functionally interact to determine vesicle luminal composition. PMID: 15073168
  26. volume-regulated ClC-3 Cl(-) channels play important role in the regulation of [Cl(-)](i) and cell proliferation of vascular smooth muscle cells PMID: 15596438
  27. Clcn3 was considered the most likely candidate of Cl- channel involved in volume regulation of human sperm. PMID: 16033995
  28. ClC-3 is required for normal neutrophil oxidative function, phagocytosis, and transendothelial migration PMID: 16522634
  29. This study demonstrates that superoxide flux across the endothelial cell plasma membrane occurs through chloride channel-3 channels and induces intracellular calcium release, which activates mitochondrial superoxide generation. PMID: 17360969
  30. The relative density of CLC-3 mRNA was 0.22+/-0.09 and 0.12+/-0.05 in HNECs treated with 3 and 0.9% saline, respectively. PMID: 17869465
  31. CLC-3 is upregulated in ethmoid mucosa and may affect the development of chronic rhinosinusitis without nasal polyps. PMID: 17882904
  32. inhibition of the NADPH oxidase or ClC-3 in otherwise unstimulated cells elicited a phenotype similar to that seen after endotoxin priming, suggesting that basal oxidant production helps to maintain cellular quiescence. PMID: 17908687
  33. oxidation induces surface expression of ClC-3 and activation of a ClC-3-dependent anion permeability in K562 cells PMID: 17976378
  34. ClC-3 overexpression induced a pH-sensitive conductance in HEK293T cells that is biophysically similar to ClC-4 and ClC-5. PMID: 17977943
  35. These data confirm that ClC-3 is important in VRAC function and cell volume regulation, is associated with the I(Cl,LPA) current activity, and participates in the fibroblast-to-myofibroblast transition. PMID: 18077605
  36. An essential role of sClC-3 in native volume-sensitive outwardly rectifying anion channels function in mouse atrial myocytes. PMID: 18986326

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Subcellular Location
[Isoform 1]: Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.; [Isoform 2]: Golgi apparatus membrane; Multi-pass membrane protein. Cell projection, ruffle membrane; Multi-pass membrane protein.
Protein Families
Chloride channel (TC 2.A.49) family, ClC-3/CLCN3 subfamily
Tissue Specificity
Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic
Database Links

HGNC: 2021

OMIM: 600580

KEGG: hsa:1182

STRING: 9606.ENSP00000261514

UniGene: Hs.481186

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