Recombinant Shewanella sp. Electron transport complex protein RnfE (rnfE)

Code CSB-CF368578STS
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Source in vitro E.coli expression system
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Product Details

Target Names
Shewana3_2173
Uniprot No.
Alternative Names
rnfE; Shewana3_2173; Ion-translocating oxidoreductase complex subunit E; Rnf electron transport complex subunit E
Species
Shewanella sp. (strain ANA-3)
Expression Region
1-232
Target Protein Sequence
MTNYREIAWQGLWKNNPGLVQLLGLCPLLAVTATLTNALGLGVATMLVLIGSNILVSLVR DYVPKEIRIPVFVMIIAALVTAVQLLINAYAYGLYLSLGIFLPLIVTNCIIIGRAEAFAS RNNAFSAAFDGLMMGLGFTLVLAVLGATREILGQGTLFDGADQLLGPWAKALTIQVWQVD TPFLLAMLPPGAFIVMGLLIALKNVIDKKLKERQPEVAVQPSVTRARITKVS
Protein Length
full length protein
Tag Info
The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane.
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
NqrDE/RnfAE family
Database Links
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7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
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