Molecular Weight Calculator

Molecular weight, also known as molecular mass, is an important parameter of DNA, RNA, protein and formula. The molecular weight of water is 18.02 g/mol, while it could be millions for many complex organic molecules. These calculators are useful and user-friendly research tools offering the relative professional and exact datas. You can not miss it if you work in the field of Molecular Biology and Chemistry. Do not hesitate to contact us if you have any questions or suggestions.


DNA Molecular Weight Calculator

DNA molecular weight is an important parameter for Molecular Biology. When you are performing a PCR or running a gel or constructing a recombinant vector, it is essential to know the DNA molecular weight of the samples. This calculator could figure out the DNA molecular weight of one or multiple FASTA sequences simultaneously which are entered directly or uploaded with txt or fasta file extension. Additionally, it gives the GC content, moles and copies of ssDNA you enter which are also useful to determine characteristics of DNA.

Note: Sequences are treated as sigle-stranded linear DNA with a 5' phosphate. Each one of the multiple FASTA sequences starting with ">" , Peptide or Protein sequences is written one by one. No more than 3,000,000 characters are recommended.

Or upload them with txt or fasta file extension 
Result MWsense
(g/mol)
MWanti-sense
(g/mol)
MWboth
(g/mol)
GC
content
Moles
(pmol/μg)
Copies/
μg

Note: MWsense, MWanti-sense and MWboth represents molecular weight of sense strand, anti-sense strand and double strand respectively.


RNA Molecular Weight Calculator

RNA molecular weight is an important parameter for Molecular Biology. When you are performing a PCR or running a gel, it is essential to know the RNA molecular weight of the samples. This calculator could figure out the RNA molecular weight of one or multiple FASTA sequences simultaneously which are entered directly or uploaded with txt or fasta file extension. Additionally, it gives the GC content, moles and copies of ssRNA you enter which are also useful to determine characteristics of RNA.

Note: Sequences are treated as sigle-stranded linear RNA with a 5' triphosphate. Each one of the multiple FASTA sequences starting with ">" , Peptide or Protein sequences is written one by one. No more than 3,000,000 characters are recommended.

Or upload them with txt or fasta file extension 
Result MWsense
(g/mol)
MWanti-sense
(g/mol)
MWboth
(g/mol)
GC
content
Moles
(pmol/μg)
Copies/
μg

Note: MWsense, MWanti-sense and MWboth represents molecular weight of sense strand, anti-sense strand and double strand respectively.


Peptide and Protein Molecular Weight Calculator

Peptide or protein molecular weight is an important parameter for Molecular Biology. When you are isolating protein, purifying protein or analyzing concentration of protein, it is essential to know the peptide or protein molecular weight of the samples. This calculator could figure out the peptide or protein molecular weight of one or multiple FASTA sequences simultaneously which are entered directly or uploaded with txt or fasta file extension.

Note: This calculator provides the mw of an unmodified oligopeptide or protein. Each one of the multiple FASTA sequences starting with ">" , Peptide or Protein sequences is written one by one. No more than 3,000,000 characters are recommended.

Or upload them with txt or fasta file extension 
Result MW(g/mol)

Note: MW represents peptide or protein molecular weight.


Formula Weight Calculator

Formula weight is an important parameter for Chemistry, Biochemistry and Molecular Biology. When you are preparing a solution, it is essential to know the formula weight of the components. This calculator could figure out the weight of chemical elements you enter in order. Additionally, you can export the result into an Excel spreadsheet.

Note: Pay attention to the format of inputting characters, you should input it with uppercase or lowercase format. The number of element or crystal water must be an integer or decimal which is written behind them. For example, CaSO4(H2O)1/2 and CaSO4.1/2H2O must be written as CaSO4(H2O)0.5 , and Fe3(Fe(CN)6)2 must be written as Fe5C12N12 or Fe3(FeC6N6)2 without nested parenthesis.

Or upload them with txt file extension 
Result MW(g/mol)

Note: MW represents formula weight.


Relative equations:

MWssDNA= NAxMWA+NTxMWT+NCxMWC+NGxMWG+79

Note: The molecular weight of A, T, C, G equals to 313.2, 304.2, 289.2 and 329.2 g/mol respectively. The additional 79 refers to the existence of a 5'phosphate residue.

MWssRNA= NAxMWA+NUxMWU+NCxMWC+NGxMWG+159

Note: The molecular weight of A, U, C, G equals to 329.2, 306.2, 305.2 and 345.2 g/mol respectively. The additional 159 refers to the existence of a 5'triphosphate residue.

Copies=NAxMoles

Note: NA refers to the Avogadro's constant which value is 6.02x10^23.

MWpeptide or protein=AnxMWA+RnxMWR+NnxMWN+…+VnxMWV

Note: An, Rn, Nn, Vn means the number of Alanine, Arginine, Asparagine and Valine respectively. The molecular weight of A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W and Y equals to 71.08, 103.14, 115.09, 129.12, 147.18, 57.06, 137.15, 113.17, 128.18, 113.17, 131.21, 114.11, 97.12, 128.41, 156.20, 87.08, 101.11, 99.14, 186.21 and 163.18 respectively.

ε=(5500xNW+1490xNY+125xNC)M-1 cm-1

Note: Tryptophan, Tyrosine and Cysteine widely exist in protein. The extinction coefficient of Tryptophan, Tyrosine, Cysteine equals to 5500, 1490 and 125 M-1 cm-1 respectively. The sum of these three kinds of amino acid represents the extinction coefficient of protein.

MWformula= N1xMW1+N2xMW2+N3xMW3+…+NnxMWn

Note: Nn means the number of relative element. The molecular weight of elements refers to periodic table of elements.

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