Dear researchers, we know you must have lots of work to do for your research. Considering about it, there is a sweet guy in my company developing this buffer calculator online so that you have no worries on buffer calculating. It can save your time on the process of preparing experiment. The buffer calculator can calculate various buffers which used to do experiment, including PBS Buffer, Acetic Acid-Sodium Acetate Buffer, Barbitone Sodium-HCl Buffer, Barbiturate Buffer, Borax-NaOH Buffer, Phosphate Buffer, Barbiturate Buffer, Glycine-HCl Buffer, Tris-HCl Buffer, KH2PO4-NaOH Buffer, et al.
Even though most products pages of antibodies, proteins or other reagents will list the formula, changes are everywhere. You always need a tool to deal with them.
In addition, we have launched three research tools in succession, involving reconstitution/ molarity/dilution calculator, molecular weight calculator and ELISA data analysis.
You really should have a try. Any suggestions are warmly welcome.
Here are some common buffers you may use for your experiments.
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Sodium Acetate, MW: 136.09 g/mol) | |
B (Acetic Acid, MW: 60.05 g/mol) |
Input buffer volume, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Barbitone Sodium, MW: 206.18 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) |
Input buffer volume, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Barbitone , MW: 184.19 g/mol) | |
B (Barbitone Sodium , MW: 206.18 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Borax, MW: 381.43 g/mol) | |
B (Sodium Hydroxide, MW: 40.00 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Borax, MW: 381.43 g/mol) | |
B (Boric Acid, MW: 61.84 g/mol) |
Input buffer volume, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Citric Acid, MW: 210.14 g/mol) | |
B (Sodium Hydroxide, MW: 40.00 g/mol) | |
C (Hydrochloric Acid, MW: 36.46 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Citric Acid, MW: 210.14 g/mol) | |
B (Sodium Citrate, MW: 294.12 g/mol) |
Input buffer volume, molar concentration to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (HEPES, MW: 238.3 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Glycine, MW: 75 .07 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Glycine, MW: 75 .07 g/mol) | |
B (Sodium Hydroxide, MW: 40.00 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Monopotassium Phosphate, MW: 136.09 g/mol) | |
B (Sodium Hydroxide, MW: 40.00 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Sodium Carbonate, MW: 286.20 g/mol) | |
B (Sodium Bicarbonate, MW: 84.0 g/mol) |
Input buffer volume, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Disodium Hydrogen Phosphate, MW: 141.96 g/mol) | |
B (Sodium Dihydrogen Phosphate, MW: 119.98 g/mol) | |
C (Sodium Chloride, MW: 58.44 g/mol) |
Input buffer volume, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Disodium Hydrogen Phosphate, MW: 141.96 g/mol) | |
B (Citric Acid, MW: 210.14 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Potassium Hydrogen Phthalate, MW: 204.23 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) |
Input buffer volume, concentrated multiple to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Tris, MW: 121.14 g/mol) | |
B (Edetate Disodium, MW: 372.24 g/mol) | |
C (Glacial Acetic Acid, MW: 60.05 g/mol) |
Input buffer volume, concentrated multiple to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Tris, MW: 121.14 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) | |
C (Sodium Chloride, MW: 58.44 g/mol) | |
M (Tween20, MW: 1227.5 g/mol) |
Input buffer volume, concentrated multiple, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Tris, MW: 121.14 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) | |
C (EDTA, MW: 292.24 g/mol) |
Input buffer volume, concentrated multiple to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Tris, MW: 121.14 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) | |
C (EDTA, MW: 292.24 g/mol) | |
M (Sodium Chloride, MW: 58.44 g/mol) |
Input buffer volume, molar concentration, pH to get formula.
Table 1. Required components
Component | Mass (g) |
---|---|
A (Tris, MW: 121.14 g/mol) | |
B (Hydrochloric Acid, MW: 36.46 g/mol) |