Buffer Calculator


Acetic Acid-Sodium Acetate Buffer

Stocks solutions
  • A: Sodium Acetate (CH3COONa3H2O MW: 136.09 g/mol)
  • B: Acetic Acid (CH3COOH MW: 60.05 g/mol)
  • C: Distilled water
To prepare L of Acetic Acid-Sodium Acetate Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Sodium Acetate, MW: 136.09 g/mol)
B (Acetic Acid, MW: 60.05 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Sodium Acetate to the solution.
  • Add g of Acetic Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Barbitone Sodium-HCl Buffer

Stocks solutions
  • A: Barbitone Sodium (C8H11N2NaO3 MW: 206.18 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: Distilled water
To prepare L of Barbitone Sodium-HCl Buffer (pH )

Input buffer volume, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Barbitone Sodium, MW: 206.18 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Barbitone Sodium to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Barbiturate Buffer

Stocks solutions
  • A: Barbitone (C8H12N2O3 MW: 184.19 g/mol)
  • B: Barbitone Sodium (C8H11N2NaO3 MW: 206.18 g/mol)
  • C: Distilled water
To prepareL of Barbiturate Buffer (pH )

Input buffer volume, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Barbitone , MW: 184.19 g/mol)
B (Barbitone Sodium , MW: 206.18 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Barbitone to the solution.
  • Add g of Barbitone Sodium to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Borax-NaOH Buffer

Stocks solutions
  • A: Borax (Na2B4O710H2O MW: 381.43 g/mol)
  • B: Sodium Hydroxide (NaOH MW: 40.00 g/mol)
  • C: Distilled water
To prepareL of Borax-NaOH Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Borax, MW: 381.43 g/mol)
B (Sodium Hydroxide, MW: 40.00 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Borax to the solution.
  • Add g of Sodium Hydroxide to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Boric Acid-Borax Buffer

Stocks solutions
  • A: Borax (Na2B4O710H2O MW: 381.43 g/mol)
  • B: Boric Acid (H3BO3 MW: 61.84 g/mol)
  • C: Distilled water
To prepareL of Boric Acid-Borax Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Borax, MW: 381.43 g/mol)
B (Boric Acid, MW: 61.84 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Borax to the solution.
  • Add g of Boric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Citrate-NaOH-HCl Buffer

Stocks solutions
  • A: Citric Acid (C6H8O7H2O MW: 210.14 g/mol)
  • B: Sodium Hydroxide (NaOH MW: 40.00 g/mol)
  • C: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • D: Distilled water
To prepareL of Citrate-NaOH-HCl Buffer (pH )

Input buffer volume, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Citric Acid, MW: 210.14 g/mol)
B (Sodium Hydroxide, MW: 40.00 g/mol)
C (Hydrochloric Acid, MW: 36.46 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Citric Acid to the solution.
  • Add g of Sodium Hydroxide to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Citrate-Sodium Citrate Buffer

Stocks solutions
  • A: Citric Acid (C6H8O7H2O MW: 210.14 g/mol)
  • B: Sodium Citrate (Na3C6H5O72H2O MW: 294.12 g/mol)
  • C: Distilled water
To prepareL of Citrate-Sodium Citrate Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Citric Acid, MW: 210.14 g/mol)
B (Sodium Citrate, MW: 294.12 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Citric Acid to the solution.
  • Add g of Sodium Citrate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

HEPES Buffer

Stocks solutions
  • A: HEPES (C8H18N2O4S MW: 238.3 g/mol)
  • B: Distilled water
To prepareL of HEPES Buffer ( M, pH 6.8~8.2 )

Input buffer volume, molar concentration to get formula.

Table 1. Required components

Component Mass (g)
A (HEPES, MW: 238.3 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of HEPES to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Glycine-HCl Buffer

Stocks solutions
  • A: Glycine (C2H5NO2 MW: 75 .07 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: Distilled water
To prepareL of Glycine-HCl Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Glycine, MW: 75 .07 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Glycine to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Glycine-NaOH Buffer

Stocks solutions
  • A: Glycine (C2H5NO2 MW: 75 .07 g/mol)
  • B: Sodium Hydroxide (NaOH MW: 40.00 g/mol)
  • C: Distilled water
To prepareL of Glycine-NaOH Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Glycine, MW: 75 .07 g/mol)
B (Sodium Hydroxide, MW: 40.00 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Glycine to the solution.
  • Add g of Sodium Hydroxide to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

KH2PO4-NaOH Buffer

Stocks solutions
  • A: Monopotassium Phosphate (KH2PO4 MW: 136.09 g/mol)
  • B: Sodium Hydroxide (NaOH MW: 40.00 g/mol)
  • C: Distilled water
To prepareL of KH2PO4-NaOH Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Monopotassium Phosphate, MW: 136.09 g/mol)
B (Sodium Hydroxide, MW: 40.00 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Monopotassium Phosphate to the solution.
  • Add g of Sodium Hydroxide to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Na2CO3-NaHCO3 Buffer

Stocks solutions
  • A: Sodium Carbonate (Na2CO310H2O MW: 286.20 g/mol)
  • B: Sodium Bicarbonate (NaHCO3 MW: 84.0 g/mol)
  • C: Distilled water
To prepareL of Na2CO3-NaHCO3 Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Sodium Carbonate, MW: 286.20 g/mol)
B (Sodium Bicarbonate, MW: 84.0 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Sodium Carbonate to the solution.
  • Add g of Sodium Bicarbonate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

PBS Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 MW: 141.96 g/mol)
  • B: Sodium Dihydrogen Phosphate (NaH2PO4 MW: 119.98 g/mol)
  • C: Sodium Chloride (NaCl MW: 58.44 g/mol)
  • D: Distilled water
To prepareL of PBS Buffer ( pH )

Input buffer volume, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Disodium Hydrogen Phosphate, MW: 141.96 g/mol)
B (Sodium Dihydrogen Phosphate, MW: 119.98 g/mol)
C (Sodium Chloride, MW: 58.44 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Disodium Hydrogen Phosphate to the solution.
  • Add g of Sodium Dihydrogen Phosphate to the solution.
  • Add g of Sodium Chloride to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Phosphate Buffer

(1)Na2HPO4-NaH2PO4 Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO42H2O MW: 178.05 g/mol)
  • B: Sodium Dihydrogen Phosphate (NaH2PO42H2O MW: 156.01 g/mol)
  • C: Distilled water
To prepareL of Na2HPO4-NaH2PO4 Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Disodium Hydrogen Phosphate, MW: 178.05 g/mol)
B (Sodium Dihydrogen Phosphate, MW: 156.01 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Disodium Hydrogen Phosphate to the solution.
  • Add g of Sodium Dihydrogen Phosphate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

(2)Na2HPO4-KH2PO4 Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO42H2O MW: 178.05 g/mol)
  • B: Monopotassium Phosphate (KH2PO4 MW: 136.09 g/mol)
  • C: Distilled water
To prepareL of Na2HPO4-KH2PO4 Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Disodium Hydrogen Phosphate, MW: 178.05 g/mol )
B (Monopotassium Phosphate, MW: 136.09 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Disodium Hydrogen Phosphate to the solution.
  • Add g of Monopotassium Phosphate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Phosphate-Citrate Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 MW: 141.96 g/mol)
  • B: Citric Acid (C6H8O7H2O MW: 210.14 g/mol)
  • C: Distilled water
To prepareL of Phosphate-Citrate Buffer ( pH )

Input buffer volume, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Disodium Hydrogen Phosphate, MW: 141.96 g/mol)
B (Citric Acid, MW: 210.14 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Disodium Hydrogen Phosphate to the solution.
  • Add g of Citric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Phthalate-HCl Buffer

Stocks solutions
  • A: Potassium Hydrogen Phthalate (KHC8H4O4 MW: 204.23 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: Distilled water
To prepareL of Phthalate-HCl Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Potassium Hydrogen Phthalate, MW: 204.23 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Potassium Hydrogen Phthalate to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

TAE Buffer

Stocks solutions
  • A: Tris (C4H11NO3 MW: 121.14 g/mol)
  • B: Edetate Disodium (C10H14N2Na2O82H2O MW: 372.24 g/mol)
  • C: Glacial Acetic Acid (CH3COOH MW: 60.05 g/mol)
  • M: Distilled water
To prepareL of TAE Buffer ( ×, pH: 8.0 )

Input buffer volume, concentrated multiple to get formula.

Table 1. Required components

Component Mass (g)
A (Tris, MW: 121.14 g/mol)
B (Edetate Disodium, MW: 372.24 g/mol)
C (Glacial Acetic Acid, MW: 60.05 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Tris to the solution.
  • Add g of Edetate Disodium to the solution.
  • Add g of Glacial Acetic Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

TBST Buffer

Stocks solutions
  • A: Tris (C4H11NO3 MW: 121.14 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: Sodium Chloride (NaCl MW: 58.44 g/mol)
  • M: Tween20 (C58H114O26 MW: 1227.5 g/mol)
  • N: Distilled water
To prepareL of TBST Buffer ( ×, pH: 7.5 )

Input buffer volume, concentrated multiple to get formula.

Table 1. Required components

Component Mass (g)
A (Tris, MW: 121.14 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
C (Sodium Chloride, MW: 58.44 g/mol)
M (Tween20, MW: 1227.5 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Tris to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Add g of Sodium Chloride to the solution.
  • Add g of Tween20 to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

TE Buffer

Stocks solutions
  • A: Tris (C4H11NO3 MW: 121.14 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: EDTA (C10H16N2O8 MW: 292.24 g/mol)
  • M: Distilled water
To prepareL of TE Buffer ( ×, pH )

Input buffer volume, concentrated multiple, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Tris, MW: 121.14 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
C (EDTA, MW: 292.24 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Tris to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Add g of EDTA to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

TEN Buffer

Stocks solutions
  • A: Tris (C4H11NO3 MW: 121.14 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: EDTA (C10H16N2O8 MW: 292.24 g/mol)
  • M: Sodium Chloride (NaCl MW: 58.44 g/mol)
  • N: Distilled water
To prepareL of TEN Buffer ( ×, pH: 7.5 )

Input buffer volume, concentrated multiple to get formula.

Table 1. Required components

Component Mass (g)
A (Tris, MW: 121.14 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
C (EDTA, MW: 292.24 g/mol)
M (Sodium Chloride, MW: 58.44 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Tris to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Add g of EDTA to the solution.
  • Add g of Sodium Chloride to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

Tris-HCl Buffer

Stocks solutions
  • A: Tris (C4H11NO3 MW: 121.14 g/mol)
  • B: Hydrochloric Acid (HCl MW: 36.46 g/mol)
  • C: Distilled water
To prepareL of Tris-HCl Buffer ( M, pH )

Input buffer volume, molar concentration, pH to get formula.

Table 1. Required components

Component Mass (g)
A (Tris, MW: 121.14 g/mol)
B (Hydrochloric Acid, MW: 36.46 g/mol)
Steps:
  • Prepare mL of distilled water in a suitable container.
  • Add g of Tris to the solution.
  • Add g of Hydrochloric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH.
  • Add distilled water until volume is L.

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