﻿Reconstitution / Molarity / Dilution Calculator - Cusabio

# Reconstitution (Concentration) Calculator / Molarity / Dilution Calculator

These research tools are related to some common parameters used in material reconstitution. Many researchers use this tool when doing experiments related to molecules or proteins. With the help of Reconstitution Calculator, Molarity Calculator and Dilution Calculator, you can constitute your vial from powder or stock solution easily. The principles and equations of them are basic but useful and professional. Do not hesitate to contact us if you have any questions or suggestions.

## Reconstitution (Concentration) Calculator

Many reagents are always used in a form of solution rather than solid. You can prepare a solution from dry or lyophilized powder by simply considering the mass, volume and final concentration to reconstitute your vial. Enter any two of these three parameters and the calculator will determine the rest.

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An example for CUSABIO Reconstitution Calculator:
What volume of soluent is needed to make a 10 g/L solution from 1 g solute?
a. Please enter 10 into the Concentration box and select the corresponding unit(g/L).
b. Please enter 1 into the Mass box and select the corresponding unit(g).
c. Please select the correct unit of Volume(L).
d. Press calculate, then the answer of 0.1 will come out in the Volume box.

## Molarity Calculator

Molarity is short for molar concentration, which is a common measurement of concentration depending on temperature. The formula is related to molarity, mass, volume and molecular weight. Through this calculator, you can get the result easily after entering any three of these four parameters.

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An example for CUSABIO Molarity Calculator:
What volume of soluent is needed to make a 500 g/mol solution from 5 g of solute given the molarity of the solution is 0.1 mol/L?
a. Please enter 0.1 into the Molarity box and select the corresponding unit(mol/L).
b. Please enter 5 into the Mass box and select the corresponding unit(g).
c. Please enter 500 into the Molecular wight box and select the corresponding unit(g/mol).
d. Please select the correct unit of Volume(L).
e. Press calculate, then the answer of 0.1 will come out in the Volume box.

## Dilution Calculator

Dilution is the process of making a lower concentration solution by adding more solvent to the higher one without changing the quantity of solute and it is a common step of the experiments. You can use one-step calculator to achieve initial volume, initial molarity, final volume and final molarity by simply entering any three of these four parameters and choosing their corresponding units. Additionally, a serial dilution, which is required for making a calibration curve, generates a sequence of dilutions from the initial molar concentration using the same dilution factor. The serial dilution calculator is convenient for us to have these parameters based on initial molarity and dilution rate.

One-Step Dilution

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An example for CUSABIO One-Step Dilution Calculator:
What volume of a given 10 mol/L initial solution is needed to make 2L of a 0.1 mol/L final solution?
a. Please enter 10 into the Initial Molarity box (Molarity0) and select the corresponding unit(mol/L).
b. Please enter 0.1 into the Final Molarity box (Molarity1) and select the corresponding unit(mol/L).
c. Please enter 2 into the Final Volume box and select the corresponding unit(L).
d. Please select the correct unit of Initial Volume(L).
e. Press calculate, then the answer of 0.02 will come out in the Initial Volume box.

Serial Dilution

 Initial Molarity: Dilution Rate: Molarity1 Molarity2 Molarity3 Molarity4 Molarity5 Molarity6 Molarity7 Molarity8

An example for CUSABIO Serial Dilution Calculator:
What is the sequence of molarity from a 512 mol/L initial solution given the dilution factor is 2?
a. Please enter 512 into the Initial Molarity box (Molarity0).
b. Please enter 2 into the Dilution Rate box (Factor).
c. Press calculate, then the answer of 256, 128, 64, 32, 16, 8, 4, 2 will come out in the right box.

Relative equations:

Mass=Concentration x Volume

Note: Mass is the amount of matter present in a substance which value is constant unaffected by gravity.

Mass=Molecular weight x Volume x Molarity

Note: Molarity is the amount of a solute per volume unit of a solution which value is affected by temperature..

Molarity0 x Volume0 =Molarity1 x Volume1

Note: Molarity0, Volume0, Molarity1 and Volume1 represents initial molarity, initial volume, final molarity and final volume respectively.

Molarityn=Molarity0÷R^n

Note: Molarity0 and R represents initial molarity and dilution rate respectively.