Product Details

Target Name

CD163 molecule

Alternative Names

CD163 ELISA Kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA Kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA Kit; sCD163)] ELISA Kit

Abbreviation

CD163

Uniprot No.

Q2VL90

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

93.75 ng/mL-6000 ng/mL

Sensitivity

23.4 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-90
1:2	Average %	94
	Range %	91-97
1:4	Average %	103
	Range %	100-106
1:8	Average %	99
	Range %	95-103

Recovery

The recovery of pig CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	89	86-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
6000	2.111	2.228	2.170	2.003
3000	1.365	1.455	1.410	1.243
1500	0.845	0.868	0.857	0.690
750	0.555	0.574	0.565	0.398
375	0.369	0.349	0.359	0.192
187.5	0.282	0.263	0.273	0.106
93.75	0.235	0.230	0.233	0.066
0	0.170	0.163	0.167

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Pig CD163 ELISA Kit was designed for the quantitative measurement of Pig CD163 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 ng/mL-6000 ng/mL and the sensitivity is 23.4 ng/mL.

Target Background

Function
(From Uniprot)

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. May play a role in the process of infection of porcine monocytes/macrophages by African swine fever virus (ASFV). In case of porcine reproductive and respiratory syndrome virus (PRRSV), serves mediates virion attachment and plays a role in viral entry.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role.

Gene References into Functions

Study investigated the association of three CD163 polymorphisms with host immune responses following PRRSV challenge and also analyzed the combined effects of the CD163 SNPs. The most significant associations were found with the c.2509G>C, c.2638A>G and c.3534C>T polymorphisms. PMID: 29285769
deletion of Exon 7 of the CD163 gene, encoding SRCR5 had no adverse effects in pigs and these animals showed complete resistance to viral infection by the positive-strand RNA PRRS virus PMID: 28231264
CD163 is not necessary for infection with African swine fever virus. PMID: 27898335
CD163 represents an additional candidate gene for resistance against porcine reproductive and respiratory syndrome. PMID: 26711047
Overexpression of ADAM17 induced downregulation of CD163 expression and a reduction in reproductive and respiratory syndrome virus infection. PMID: 24965453
Fusion protein of sialoadhesin and domains 5-9 of CD163 receptors blocked the respiratory syndrome virus infection. PMID: 24246307
GP4 is involved in interaction with cellular receptor CD163. PMID: 22537809
). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs. PMID: 21786159
Porcine reproductive and respiratory syndrome virus GP4 was found to co-localize with CD163 in the lipid rafts on the plasma membrane. PMID: 22222209
Our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during porcine reproductive and respiratory syndrome virus replication. PMID: 20496088
The newly established porcine alveolar macrophage cell lines were demonstrated to express robust levels of CD163 and to be fully permissive for both type 1 and 2 porcine reproductive and respiratory syndrome virus strains. PMID: 19900480
The carboxy-terminal 223 residues of the CD163 molecule are not required for interactions with either the GP2a or the GP4 protein, although these residues are required for conferring susceptibility to PRRSV infection. PMID: 19939927
CD163 is shown to be involved in PRRSV (porcine reproductive and respiratory syndrome virus) entry, probably during uncoating, and to interact with sialoadhesin in the process. PMID: 19008379
The expression level of CD163 correlates with the level of PRRSV infection. PMID: 19111584
Clear changes in the quantity of sialoadhesin(+) and CD163(+) macrophages in the placentas and organs of embryos/fetuses during gestation. PMID: 19410291

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Subcellular Location

[Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed in monocytes and macrophages. Detected only in one population of monocytes (CD163+) which is in advanced maturation stage.

Database Links

KEGG: ssc:397031

UniGene: Ssc.100404

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Pig Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit

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