Product Details

Target Name

low density lipoprotein receptor

Alternative Names

Ldlr ELISA Kit; Low-density lipoprotein receptor ELISA Kit; LDL receptor ELISA Kit

Abbreviation

LDLR

Uniprot No.

P35952

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDLR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	85
	Range %	80-89
1:400	Average %	102
	Range %	98-108
1:800	Average %	90
	Range %	85-94
1:1600	Average %	97
	Range %	92-101

Recovery

The recovery of rat LDLR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.512	2.475	2.494	2.355
250	2.247	2.196	2.222	2.083
125	1.803	1.689	1.746	1.607
62.5	1.189	1.227	1.208	1.069
31.2	0.687	0.713	0.700	0.561
15.6	0.415	0.392	0.404	0.265
7.8	0.298	0.289	0.294	0.155
0	0.136	0.141	0.139

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat LDLR ELISA Kit was designed for the quantitative measurement of Rat LDLR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target Background

Function
(From Uniprot)

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

Gene References into Functions

LDLR is a relevant receptor for CNS drug delivery via receptor-mediated transcytosis and that the peptide vectors we developed have the potential to transport drugs PMID: 28108572
Intrauterine growth restriction combined with a maternal high-fat diet increases hepatic cholesterol accumulation via increased LDL cholesterol uptake into the liver with resulting persistent increases in hepatic cholesterol accumulation. PMID: 27401460
Compared with the cholesterol-enriched restructured pork group, silicon-enriched restructured pork rats had lower VLDL compound concentrations and lower VLDL oxidation but greater Ldlr expression. PMID: 26246324
The LDL receptor could be located at once in clathrin-coated pits and caveolae. PMID: 24530906
LDLR mutant rats are a useful novel animal model of hypercholesterolemia and atherosclerosis. PMID: 22293196
A tissue-specific dysregulation of LDLr is demonstrated that is age- and gender-dependent. PMID: 21792919
expression of LDLR was increased in the hippocampus of stroke-prone spontaneously hypertensive rats and was seen in the luminal and abluminal cytoplasmic membranes and vesicular structures of endothelial cells PMID: 20458492
expression pattern of LDL-R in brain microvessels endothelial cells and its modulation by astrocytes PMID: 12095612
cloning and sequencing of the rat LDL receptor 5'-flanking region revealed the presence of several putative cis-acting elements PMID: 16647292
Retina is capable of rapid uptake of circulating LDL via LDLR-mediated process primarily occurring in retinal pigment epithelium and also possibly Muller cells. PMID: 17110914
Caloric restriction affects Ldlr involved in age-related hypercholesterolemia. PMID: 17203467
LDLR-mediated pathway was involved in the endocytosis of Poly(methoxypolyethyleneglycol cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles by RBEC. PMID: 17256088
Modeling of corticosteroid effects on hepatic low-density lipoprotein receptors and plasma lipid dynamics in rats. PMID: 17674160

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.

Protein Families

LDLR family

Database Links

KEGG: rno:300438

STRING: 10116.ENSRNOP00000013496

UniGene: Rn.10483

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Code	CSB-E13545r
Size	96T,5×96T,10×96T
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Rat low density lipoprotein receptor,LDLR ELISA Kit

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Target Background