Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Target Names

CCL7

Uniprot No.

P80098

Research Area

Immunology

Alternative Names

C-C motif chemokine 7; Ccl7; CCL7_HUMAN; Chemokine CC motif ligand 7; FIC; MARC; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; SCYA6; SCYA7; Small-inducible cytokine A7

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-99aa

Complete Sequence

QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL

Mol. Weight

9.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM PB, pH 7.4, 150 mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Discover the potential of our Recombinant Human CCL7 protein, an indispensable tool for researchers in immunology. This C-C motif chemokine 7, also known as CCL7, MCP3, SCYA6, and SCYA7, is expressed in E. coli and comprises the 24-99aa expression region of the full-length mature protein. The tag-free protein is provided as a lyophilized powder, ensuring straightforward reconstitution with sterile water or buffer for various experimental uses.

Quality is of utmost importance to us, and our Recombinant Human CCL7 protein demonstrates a purity of >97% as determined by SDS-PAGE and HPLC analysis. Endotoxin levels are kept below 1.0 EU/µg, as assessed by the LAL method. The protein exhibits full biological activity in a chemotaxis bioassay using human monocytes, with an effective concentration range of 10-100 ng/ml.

Recent investigations have explored the role of CCL7 in immune system regulation and its involvement in numerous pathologies, including inflammatory diseases^[1], autoimmune diseases^[2], and cancer^[3]. The versatile functions of CCL7 in the immune system emphasize its importance as a research target and its potential therapeutic applications.

References:
1. Proost P, et al. Posttranslational modifications affect the activity of the human monocyte chemotactic proteins MCP-1 and MCP-2: identification of MCP-2(6-76) as a natural chemokine inhibitor. J Immunol. 1996;156(6): 2079-85.
2. Weber C, et al. MCP-3 (CCL7) delivered by parvovirus MVMp reduces tumorigenicity of mouse melanoma cells through activation of T lymphocytes and NK cells. Int J Cancer. 2002;99(6): 923-7.
3. Wang L, et al. The C-C motif chemokine ligand 7 and interferon γ play an important role in the development of focal and segmental glomerulosclerosis. Ann Transl Med. 2020;8(6): 388.

Target Background

Function

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

Gene References into Functions

Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells. PMID: 28475003
Periprostatic adipocytes drive prostate cancer progression in obesity via CCL7 secretion which stimulates CCR3 expressing tumor cells. PMID: 26756352
These results suggest that let-7d may suppress renal cell carcinoma growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7. PMID: 25193015
Based on nasopharyngeal CCL7 gene expression in readily available Nasopharyngeal aspirate samples , we can discriminate between severity of disease in Respiratory syncytial virus infected infants. PMID: 25261323
Overexpression of ISL1 in human mesnchymal stem cells promotes angiogenesis in vitro and in vivo through increasing secretion of MCP3 and other paracrine factors. PMID: 24578274
Our data demonstrate that the monocyte-specific chemokine CCL7 and its receptor CCR2 are expressed in tumour cells of RCC PMID: 24327013
Systemic IFN-gamma triggered the secretion of C-C motif ligand chemokines CCL2 and CCL7 leading to the egress of early, myeloid-committed progenitors from the bone marrow mediated by their common receptor CCR2. PMID: 23762028
Anticancer effect of desmethyl-lasiodiplodin is mediated, in part, by upregulation of apoptotic genes and downregulation of MCP-3. PMID: 23764760
Immunohistochemistry and real-time PCR analysis showed that CCL7 was expressed in normal colonic epithelium and the expression was higher in liver metastases compared to primary CRC. PMID: 22614322
These results suggest that overexpression of CCL21 and CCL7 is associated with tumor metastasis and serves as a prognostic factor in patients with gastric cancer. PMID: 22468089
MCP-3 is produced by human coronary artery smooth muscle cells and directly induces CASMC proliferation in vitro, suggesting a potential role for this chemokine in vascular pathology PMID: 21536288
we established, for the first time, a significant association of MCP3 variants with atopic asthma. PMID: 21388664
Basal monocyte migration may be facilitated by the astrocyte-derived cytokine CCL7 whose production is rapidly increased by TNF-alpha and thus likely plays a critical role in initiating neuroinvasion by SIV/HIV. PMID: 21279498
The expression of MCP-3 in laryngeal squamous cell carcinoma is much higher than in normal tissue. PMID: 18533557
IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells. PMID: 19253530
Cytoplasmic GR interacts with a subset of CCL7 mRNA through specific sequences and can regulate turnover rates, a novel posttranscriptional role for GR as an RNA-binding protein. PMID: 21148795
Monocyte chemoattractant protein-3 (MCP-3) was highly expressed in patients with chronic periodontitis, particularly in those with progressive periodontal lesions. PMID: 20151806
higher expression in the genital mucosa than in the blood of HIV-1-infected women from Benin PMID: 19898927
Elevated level of CCL7 is associated with invasion and migration of oral squamous cell carcinoma. PMID: 19937793
The two most abundant alleles of the CA/GA microsatellite polymorphism in the promoter-enhancer region of the MCP-3 gene, A2 and A3, are significantly associated with multiple sclerosis in the Belgian case-control study, but not in the family study. PMID: 12127674
overexpression of MCP-3 in early-stage systemic sclerosis suggests a novel role for this protein as a fibrotic mediator activating extracellular matrix gene expression in addition to promoting leukocyte trafficking. PMID: 12847692
monocyte chemotactic protein-3 uses a different mode of action from eotaxin PMID: 14733956
data suggest that cyclic mechanical stretch of the uterine cervix by the presenting part of the fetus during labour may augment both IL-8 and MCP-3 production in the uterine cervix via AP-1 activation. PMID: 15194816
These results suggest that oxLDL delivers its signal for MCP-3 expression via PPARgamma, which may be further related to the atherogenesis. PMID: 15381085
A non-heparin-binding mutant CCL7 inhibits chemotactic potential of synovial fluid from patients with active rheumatoid arthritis and demonstrates the glycosaminoglycan-binding requirement for chemokine-driven inflammation in vivo PMID: 16002730
CCR8 ligands are allotropic, binding to distinct sites within CCR8; the human immune system may have evolved to use CCL7 as a selective antagonist of viral chemokine activity at CCR8 but not those of the host ligand PMID: 17023422
MCP-3 is critical for monocyte mobilization and suggests new roles for monocyte chemoattractants in leukocyte homeostasis. PMID: 17364026
CCL7 synergizes with coproduced CXCL8 in peripheral blood monocyte migration. PMID: 18469140
increased plasma levels in patients with amnestic mild cognitive impairment PMID: 19403065
High MCP-3 is associated with macrophage/microglia infiltration in gliomas. PMID: 19424580