gyrA Antibody

Code CSB-PA365190XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) gyrA Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
gyrA antibody; hisW antibody; nalA antibody; parD antibody; b2231 antibody; JW2225 antibody; DNA gyrase subunit A antibody; EC antibody
Raised in
Species Reactivity
Escherichia coli (strain K12)
Recombinant Escherichia coli (strain K12) gyrA protein
Immunogen Species
Escherichia coli (strain K12)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to maintain chromosomes in an underwound state. This makes better substrates for topoisomerase IV (ParC and ParE) which is the main enzyme that unlinks newly replicated chromosomes in E.coli. Gyrase catalyzes the interconversion of other topological isomers of dsDNA rings, including catenanes. Relaxes negatively supercoiled DNA in an ATP-independent manner. E.coli gyrase has higher supercoiling activity than many other bacterial gyrases; at comparable concentrations E.coli gyrase introduces more supercoils faster than M.tuberculosis gyrase, while M.tuberculosis gyrase has higher decatenation than supercoiling activity compared to E.coli. E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical. The enzymatic differences between E.coli gyrase and topoisomerase IV are largely due to the GyrA C-terminal domain (approximately residues 524-841) and specifically the GyrA-box.; Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
Gene References into Functions
  1. analysis of gyrase mutations in E.coli that could be responsible for increased quinolone resistive mechanisms among enteric pathogens; docking studies revealed displacement of quinolone binding site in mutated protein complex which resulted in lower binding energy as compared to the normal one PMID: 29300775
  2. The present study was undertaken to better understand the dynamic behavior of the gyrA in Enterotoxigenic Escherichia coli [ETEC] and to decipher the structural changes associated with mutations, Ser83Leu and Ser83Leu/Asp87Asn, leading to ciprofloxacin antibiotic resistance in ETEC gyrA. PMID: 27753544
  3. Data indicate the existence of interactions between the fluoroquinolone C-7 ring and both GyrA and GyrB. PMID: 24497635
  4. This new structure is entirely consistent with the mutations in GyrA that confer Simocyclinone D8 resistance. PMID: 24594357
  5. The C-terminal part of McbA is crucial for DNA gyrase inhibition and antibiotic uptake. PMID: 24563033
  6. binds to plasmid-encoded quinolone resistance protein Qnr PMID: 15616284
  7. The low-resolution structure of the full-length A subunit (GyrA)was reported. PMID: 15698572
  8. Analysis of DNA supercoiling by the E. coli GyrA C-terminal domain PMID: 15897198
  9. acquisition of a fourth resistance mutation significantly increased fitness especially with the addition of a parC mutation (Topoisomerase IV) to a low-fitness strain carrying resistance mutations in gyrA (DNA Gyrase) and marR (drug efflux regulation) PMID: 19662169

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Subcellular Location
Protein Families
Type II topoisomerase GyrA/ParC subunit family
Database Links
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7505 Fannin St Ste 610-322 Houston, TX 77054, USA
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