RAP1 Antibody

Code CSB-PA319350XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAP1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
RAP1 antibody; GRF1 antibody; TUF1 antibody; YNL216W antibody; N1310 antibody; DNA-binding protein RAP1 antibody; Repressor/activator site-binding protein antibody; SBF-E antibody; TUF antibody
Raised in
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RAP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene.
Gene References into Functions
  1. Rap1 (ScRap1) can either activate or repress transcription. This bimodal transcriptional activity has led to the widespread study of the mode of action of ScRap1. This review summarizes current knowledge about ScRap1, including its structure, mechanisms of transcription regulation, and biological functions, and the future directions in the field. Review. PMID: 27100480
  2. Pif1, a 5'-3' helicase, not only stimulates the strand displacement activity of Pol delta but it also allows efficient replication through the Rap1 block, by removing bound Rap1 in front of the polymerase. PMID: 27001517
  3. that Rap1 contains an AD required for Rap1-dependent gene transcription. The Rap1(AS) variant will likely also be useful for studies of the functions of Rap1 in other biological pathways. PMID: 28196871
  4. Rap1 contributes to telomere homeostasis by promoting chromosome breakage. PMID: 26263073
  5. found that all arrangements of Rap1 sites tested are represented within the telomeric sequence and our data suggest that at telomeres Rap1 might form a nucleoprotein complex with a heterogeneous distribution of bound states PMID: 25637888
  6. Rap1 C-terminal (RCT) domain of yeast Rap1 modulate access to different DNA binding modes PMID: 25805496
  7. findings suggest a connection between Rap1p and cell wall homeostasis. PMID: 25481258
  8. while Rap1 DNA-binding domain forms a high-affinity 1:1 complex with its DNA recognition site, it can also form lower-affinity complexes with higher stoichiometries on DNA PMID: 25382181
  9. Rap1 relocalization is thus a novel mechanism connecting DNA damage responses (DDRs) at telomeres to global changes in chromatin and gene expression while driving the pace of senescence PMID: 23756653
  10. Rap1 may cause permanganate hypersensitivity of DNA by forming a pocket between the reactive cytosine and Arg580, driving the permanganate ion towards the C5-C6 bond of the cytosine. PMID: 23519416
  11. These data support a model in which Tbf1 and Rap1 collaborate to maintain genomic stability of short telomeres. PMID: 22130795
  12. Results reveal Rap1 structural adjustment upon DNA binding that involves a specific orientation of the C-terminal domain with regard to the DNA binding domain. PMID: 22139930
  13. Rap1p association with target promoter in the nucleus affects the composition of the exported mRNP, which in turn regulates mRNA decay in the cytoplasm. PMID: 22196725
  14. Results show that Rap1 contributes to local regions of chromatin structure by acting at both strong and weak binding sites, at proximal promoter regions and at sites farther upstream, over a very large fraction of the yeast genome. PMID: 21081559
  15. Structural comparison indicated that Rap1 BRCT domain adopts a global fold similar to other BRCT domains, implying some common structural aspects of BRCT domain family. PMID: 21187076
  16. Data suggest that Rap1 binding to multiple sites in a telomere array does not, by itself, promote formation of a more energetically stabile complex. PMID: 20826803
  17. Rap1 is essential to block nonhomologous end joining between telomeres PMID: 16096640
  18. The effect on global gene expression of loss of binding of Rap1. PMID: 17158163
  19. Rap1 binding is required for end concealment at long tracts and modulates telomerase access at chromosome ends. PMID: 17289918
  20. Rap1p represents a heretofore unrecognized regulator of TATA-binding protein PMID: 18195009
  21. Structural and functional studies of the RAP1 C-terminus reveal novel separation-of-function mutants. PMID: 18538788

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Subcellular Location
Nucleus. Chromosome, telomere.
Protein Families
RAP1 family
Database Links

KEGG: sce:YNL216W

STRING: 4932.YNL216W

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