REV1 Antibody

Code CSB-PA019576XA01SVG
Size Enquire
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REV1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
REV1 antibody; YOR346W antibody; O6339 antibody; DNA repair protein REV1 antibody; EC 2.7.7.- antibody; Reversionless protein 1 antibody
Raised in
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) REV1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.
Gene References into Functions
  1. The structures of the translesion DNA synthesis polymerase Rev1 in complex with benzo[a]pyrene guanine adducts gives insights how Rev1 achieves error-free replication. PMID: 29042535
  2. Rev1-BRCT domain bound to PCNA can be displaced by increasing amounts of the PIP-box peptide. PMID: 23747975
  3. Rev1 stably interacts with Rad5 via the C-terminal region of Rev1 and the N-terminal region of Rad5. PMID: 23142547
  4. The study reports the X-ray crystal structure of the yeast Rev1 BRCT domain and show that substitutions in residues constituting its phosphate-binding pocket do not affect mutagenic translesion synthesis. PMID: 23240687
  5. the levels of Rev1 protein are dependent on proteasome-mediated degradation;proteasomal degradation acts as a regulatory system of mutagenic translesion DNA synthesis mediated by Rev1. PMID: 21227758
  6. results show that Rev1's catalytic activity is important in vivo when the cell has to cope with specific DNA lesions, such as N(2)-dG. PMID: 20980236
  7. We present here the structure of yeast Rev1 in ternary complex with DNA containing an abasic lesion and with dCTP as the incoming nucleotide. The structure shows a mechanism of synthesis across an abasic lesion that differs from that in other polymerases. PMID: 21167175
  8. Only the second ubiquitin-binding motif of Saccharomyces cerevisiae Rev1 binds to ubiquitin and is essential for Rev1-dependent cell survival and mutagenesis. PMID: 20159559
  9. Dbf4 and Rev1 BRCT domains interact with a common protein or structure, although the precise function of both domains and their binding partners remains elusive PMID: 19822727
  10. Strains deficient in REV1 protein show little bypass of the T-T (6-4) photoadduct or the abasic sites. PMID: 15520252
  11. REVIEW: REV1 is involved in somatic hypermutation and double strand break repair PMID: 15588843
  12. The polymerase domain of REV1 is responsible for the dCMP incorporation; the BRCT domain is important but dispensable for translation synthesis. PMID: 15896814
  13. crystal structure of Rev1 bound to template G and incoming 2'-deoxycytidine 5'-triphosphate (dCTP), which reveals that the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base PMID: 16195463
  14. DNA polymerase zeta and Rev1 proteins function in the mitochondria PMID: 16452144
  15. Data show that Saccharomyces cerevisiae Rev1 is subject to pronounced cell cycle control in which the levels of Rev1 protein are approximately 50-fold higher in G(2) and throughout mitosis than during G(1) and much of S phase. PMID: 16751278
  16. Results report that elevated levels of the yeast Rev1 C terminus confer a strong dominant-negative effect on viability and induced mutagenesis after DNA damage. PMID: 16923957
  17. Rev1 binding enhances the proficiency of DNA polymerase zeta for the extension of mismatched primer termini on undamaged DNAs and for the extension of primer termini opposite DNA lesions. PMID: 17030609
  18. pre-steady-state kinetic studies with Rev1p PMID: 17960914
  19. We further show that the minimal C-terminal fragment of Rev1 containing these highly conserved motifs is sufficient to interact with Rev7. PMID: 18603483
  20. Rev1 may be involved in facilitating mutagenic and recombinagenic responses to the failure of Pol delta. PMID: 18711219
  21. plays a role in mutagenesis and translesin DNA synthesis. (review) PMID: 18975621
  22. Results suggest that the proto-oncogene homologue Sch9 promotes the accumulation of superoxide-dependent DNA damage in nondividing cells by a superoxide and Rev1/Polzeta-dependent mechanism. PMID: 19687253

Show More

Hide All

Subcellular Location
Nucleus. Mitochondrion.
Protein Families
DNA polymerase type-Y family
Database Links

KEGG: sce:YOR346W

STRING: 4932.YOR346W

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Leave a message

check code
© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1