Human ADP-ribosylation factor-like protein 8B(ARL8B) ELISA kit

Code CSB-EL002100HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human ARL8B ELISA Kit was designed for the quantitative measurement of Human ARL8B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Name ADP-ribosylation factor-like 8B
Alternative Names ADP ribosylation factor like 10C ELISA Kit; ADP ribosylation factor like 8B ELISA Kit; ADP-ribosylation factor-like protein 10C ELISA Kit; ADP-ribosylation factor-like protein 8B ELISA Kit; ARL10C ELISA Kit; ARL8B ELISA Kit; ARL8B_HUMAN ELISA Kit; FLJ10702 ELISA Kit; Gie1 ELISA Kit; Novel small G protein indispensable for equal chromosome segregation 1 ELISA Kit
Abbreviation ARL8B
Uniprot No. Q9NVJ2
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ARL8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 96  
Range % 90-100  
1:10 Average % 93  
Range % 89-97  
1:20 Average % 104  
Range % 99-109  
1:40 Average % 84  
Range % 80-88  
Recovery
The recovery of human ARL8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 95-104  
EDTA plasma (n=4) 90 85-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.680 2.752 2.716 2.538  
500 1.979 2.076 2.028 1.850  
250 1.230 1.214 1.222 1.044  
125 0.773 0.739 0.756 0.578  
62.5 0.455 0.476 0.466 0.288  
31.2 0.384 0.369 0.377 0.199  
15.6 0.270 0.286 0.278 0.100  
0 0.175 0.181 0.178    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis. Critical factor involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function. Also acts as a regulator of endosome to lysosome trafficking pathways of special significance for host defense. Regulates cargo trafficking to lysosomes by binding to PLEKHM1 and recruiting the HOPS subunit VPS41, resulting in functional assembly of the HOPS complex on lysosomal membranes. Plays an important role in cargo delivery to lysosomes for antigen presentation and microbial killing. Directs the intersection of CD1d with lipid antigens in lysosomes, and plays a role in intersecting phagosomes with lysosomes to generate phagolysosomes that kill microbes. Involved in the process of MHC II presentation. Regulates the delivery of antigens to lysosomes and the formation of MHC II-peptide complexes through the recruitment of the HOPS complex to lysosomes allowing the fusion of late endosomes to lysosomes. May play a role in chromosome segregation.; (Microbial infection) During Mycobacterium tuberculosis (Mtb) infection, is required for plasma membrane repair by controlling the exocytosis of lysosomes in macrophages. ARL8B secretion pathway is crucial to control the type of cell death of the M. tuberculosis-infected macrophages, distinguishing avirulent from virulent Mtb induced necrotic cell death.; (Microbial infection) During infection, coronaviruses such as SARS-CoV-2 and the chaperone HSPA5/GRP78 are probably co-released through ARL8B-dependent lysosomal exocytic pathway for unconventional egress.
Gene References into Functions
  1. Authors present evidence that Arl8b facilitates lipid hydrolysis to maintain efficient metabolism for a proliferative capacity in low nutrient environments, suggesting a likely explanation for the complete inability of Arl8b-depleted tumor cells to grow in vivo. PMID: 27105540
  2. Data indicate that E3 ubiquitin-protein ligase RNF167 ubiquitinates Arl8B at the lysine residue K141 and reduces the level of the ADP-ribosylation factor-like 8B Arl8B protein. PMID: 27808481
  3. Arl8b regulates the association of the human HOPS complex with lysosomal membranes. PMID: 25908847
  4. a small GTP-binding protein, ADP-ribosylation factor-like 8b (Arl8b), is identified as a critical factor required for NK cell-mediated cytotoxicity. PMID: 24088571
  5. The authors find that Arl8B is required for kinesin-1 recruitment to Salmonella-containing vacuoles, migration of the vacuoles to the cell periphery 24 h after infection and for cell-to-cell transfer of bacteria to neighbouring cells. PMID: 21824248
  6. a critical regulator of cargo delivery to lysosomes PMID: 21802320
  7. Results suggest that the novel GTPases Gie1 and Gie2 associate with microtubules, and might be involved in chromosome segregation. PMID: 15331635
  8. Arl8b is involved in trafficking processes for lysosomes. PMID: 16650381
  9. Data show that with MAK3 knockdown, p53 is stabilized and phosphorylated and there is a significant transcriptional activation of proapoptotic genes downstream of p53, and that localization of Arl8b is altered, suggesting that Arl8b is a Mak3 substrate. PMID: 19398576

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Subcellular Location Late endosome membrane. Lysosome membrane. Cytoplasm, cytoskeleton, spindle. Cell projection, axon. Cell junction, synapse. Cytolytic granule membrane.
Protein Families Small GTPase superfamily, Arf family
Tissue Specificity Ubiquitously expressed.
Database Links

HGNC: 25564

OMIM: 616596

KEGG: hsa:55207

STRING: 9606.ENSP00000256496

UniGene: Hs.250009

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