ARL8B Antibody

Code CSB-PA868330LA01HU
Size US$166
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  • Western blot
    All lanes: ARL8B antibody at 4µg/ml
    Lane 1: Mouse brain tissue
    Lane 2: NIH/3T3 whole cell lysate
    Lane 3: Jurkat whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 22, 19 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA868330LA01HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ARL8B Polyclonal antibody
Uniprot No.
Target Names
ARL8B
Alternative Names
ADP ribosylation factor like 10C antibody; ADP ribosylation factor like 8B antibody; ADP-ribosylation factor-like protein 10C antibody; ADP-ribosylation factor-like protein 8B antibody; ARL10C antibody; ARL8B antibody; ARL8B_HUMAN antibody; FLJ10702 antibody; Gie1 antibody; Novel small G protein indispensable for equal chromosome segregation 1 antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human ADP-ribosylation factor-like protein 8B protein (20-186AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ARL8B Antibody (Product code: CSB-PA868330LA01HU) is Non-conjugated. For ARL8B Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA868330LB01HU ARL8B Antibody, HRP conjugated ELISA
FITC CSB-PA868330LC01HU ARL8B Antibody, FITC conjugated
Biotin CSB-PA868330LD01HU ARL8B Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis. Critical factor involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function. Also acts as a regulator of endosome to lysosome trafficking pathways of special significance for host defense. Regulates cargo trafficking to lysosomes by binding to PLEKHM1 and recruiting the HOPS subunit VPS41, resulting in functional assembly of the HOPS complex on lysosomal membranes. Plays an important role in cargo delivery to lysosomes for antigen presentation and microbial killing. Directs the intersection of CD1d with lipid antigens in lysosomes, and plays a role in intersecting phagosomes with lysosomes to generate phagolysosomes that kill microbes. Involved in the process of MHC II presentation. Regulates the delivery of antigens to lysosomes and the formation of MHC II-peptide complexes through the recruitment of the HOPS complex to lysosomes allowing the fusion of late endosomes to lysosomes. May play a role in chromosome segregation.; (Microbial infection) During Mycobacterium tuberculosis (Mtb) infection, is required for plasma membrane repair by controlling the exocytosis of lysosomes in macrophages. ARL8B secretion pathway is crucial to control the type of cell death of the M. tuberculosis-infected macrophages, distinguishing avirulent from virulent Mtb induced necrotic cell death.; (Microbial infection) During infection, coronaviruses such as SARS-CoV-2 and the chaperone HSPA5/GRP78 are probably co-released through ARL8B-dependent lysosomal exocytic pathway for unconventional egress.
Gene References into Functions
  1. Authors present evidence that Arl8b facilitates lipid hydrolysis to maintain efficient metabolism for a proliferative capacity in low nutrient environments, suggesting a likely explanation for the complete inability of Arl8b-depleted tumor cells to grow in vivo. PMID: 27105540
  2. Data indicate that E3 ubiquitin-protein ligase RNF167 ubiquitinates Arl8B at the lysine residue K141 and reduces the level of the ADP-ribosylation factor-like 8B Arl8B protein. PMID: 27808481
  3. Arl8b regulates the association of the human HOPS complex with lysosomal membranes. PMID: 25908847
  4. a small GTP-binding protein, ADP-ribosylation factor-like 8b (Arl8b), is identified as a critical factor required for NK cell-mediated cytotoxicity. PMID: 24088571
  5. The authors find that Arl8B is required for kinesin-1 recruitment to Salmonella-containing vacuoles, migration of the vacuoles to the cell periphery 24 h after infection and for cell-to-cell transfer of bacteria to neighbouring cells. PMID: 21824248
  6. a critical regulator of cargo delivery to lysosomes PMID: 21802320
  7. Results suggest that the novel GTPases Gie1 and Gie2 associate with microtubules, and might be involved in chromosome segregation. PMID: 15331635
  8. Arl8b is involved in trafficking processes for lysosomes. PMID: 16650381
  9. Data show that with MAK3 knockdown, p53 is stabilized and phosphorylated and there is a significant transcriptional activation of proapoptotic genes downstream of p53, and that localization of Arl8b is altered, suggesting that Arl8b is a Mak3 substrate. PMID: 19398576

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Subcellular Location
Late endosome membrane. Lysosome membrane. Cytoplasm, cytoskeleton, spindle. Cell projection, axon. Cell junction, synapse. Cytolytic granule membrane.
Protein Families
Small GTPase superfamily, Arf family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 25564

OMIM: 616596

KEGG: hsa:55207

STRING: 9606.ENSP00000256496

UniGene: Hs.250009

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